KIR2DL3/CD158b2 Antibody (180701) [Fluorescein]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human KIR2DL3/CD158b2 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2014F) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 180701
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

KIR2DL3/CD158b2 Antibody (180701) [Fluorescein] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human KIR2DL3/CD158b2
  Accession # P43628
• Specificity: Detects human KIR2DL3/CD158b2. Stains BaF3 cells transfected with human KIR2DL3 in flow cytometry. Does not stain BaF3 cells transfected with KIR2DL1, 2DL2, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIR2DL3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KIR2DL3/CD158b2 Antibody (180701) [Fluorescein]

• CD158 antigen-like family member B2
• CD158b2 antigen
• CD158b2
• CD158B2CD158b
• cl-6
• killer cell immunoglobulin-like receptor 2DL3
• killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3
• Killer inhibitory receptor cl 2-3
• KIR-023GB
• KIR2DL3
• KIRCL23
• KIR-K7b
• KIR-K7c
• MGC129943
• MHC class I NK cell receptor
• natural killer associated transcript 2
• natural killer cell inhibitory receptor KIR2DL3
• Natural killer-associated transcript 2
• NKAT
• NKAT2
• NKAT-2
• NKAT2A
• NKAT2B
• NKAT2GL183
• NK-receptor
• p58 natural killer cell receptor clone CL-6
• p58 NK receptor CL-6
• p58
• p58.2 MHC class-I specific NK receptor
• p58.2 MHC class-I-specific NK receptor

Background

KIR2DL3 is one of several immunoglobulin-like receptors expressed on NK and T cells that bind MHC class I molecules and regulate killer cell activity. KIR2DL3 interacts specifically with HLA-Cw3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KIR2DL3/CD158b2 Antibody (FAB2014F)(12)

Publications using FAB2014F

• Sabrina Bianca Bennstein, Sandra Weinhold, Angela Riccarda Manser, Nadine Scherenschlich, Angela Noll, Katharina Raba et al. Umbilical cord blood-derived ILC1-like cells constitute a novel precursor for mature KIR+NKG2A- NK cells eLife 7/13/2020 [PMID: 32657756]
• Samarani S, Sagala P, Jantchou P, Grimard G, Faure C, Deslandres C, Amre D, Ahmad A Phenotypic and Functional Changes in Peripheral Blood Natural Killer Cells in Crohn Disease Patients. Mediators Inflamm, 2020-02-19;2020(0):6401969. 2020-02-19 [PMID: 32148442] (Flow Cytometry, Human)
• A Pfefferle, B Jacobs, H Netskar, EH Ask, S Lorenz, T Clancy, JP Goodridge, E Sohlberg, KJ Malmberg Intra-lineage Plasticity and Functional Reprogramming Maintain Natural Killer Cell Repertoire Diversity Cell Rep, 2019-11-19;29(8):2284-2294.e4. 2019-11-19 [PMID: 31747601] (Flow Cytometry, Human)
• D Feyaerts, T Kuret, B van Cranen, S van der Ze, OWH van der He, A van der Me, I Joosten, RG van der Mo Endometrial natural killer (NK) cells reveal a tissue-specific receptor repertoire Hum. Reprod., 2018-03-01;0(0):. 2018-03-01 [PMID: 29447367] (Flow Cytometry, Human)
• AT Björklund, M Carlsten, E Sohlberg, LL Liu, T Clancy, M Karimi, S Cooley, JS Miller, M Klimkowska, M Schaffer, E Watz, KI Wikstrom, P Blomberg, BE Wahlin, M Palma, L Hansson, P Ljungman, E Hellström-, HG Ljunggren, KJ Malmberg Complete Remission with Reduction of High-risk Clones following Haploidentical NK Cell Therapy against MDS and AML Clin. Cancer Res., 2018-02-14;0(0):. 2018-02-14 [PMID: 29444931] (Flow Cytometry, Human)
• Dunphy S, Guinan K, Chorcora C, Jayaraman J, Traherne J, Trowsdale J, Pende D, Middleton D, Gardiner C 2DL1, 2DL2 and 2DL3 all contribute to KIR phenotype variability on human NK cells. Genes Immun, 2015-05-07;16(5):301-10. 2015-05-07 [PMID: 25950617] (Flow Cytometry, Human)
• Marquardt N, Beziat V, Nystrom S, Hengst J, Ivarsson M, Kekalainen E, Johansson H, Mjosberg J, Westgren M, Lankisch T, Wedemeyer H, Ellis E, Ljunggren H, Michaelsson J, Bjorkstrom N Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells. J Immunol, 2015-02-11;194(6):2467-71. 2015-02-11 [PMID: 25672754] (Flow Cytometry, Human)
• Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT. Proc Natl Acad Sci U S A, 2014-12-15;111(52):E5688-96. 2014-12-15 [PMID: 25512551] (Flow Cytometry, Human)
• Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection. Eur J Immunol, 2012-12-12;43(2):480-7. 2012-12-12 [PMID: 23161492] (Flow Cytometry, Human)
• Ordonez D, Gomez-Lozano N, Rosales L, Vilches C Molecular characterisation of KIR2DS2*005, a fusion gene associated with a shortened KIR haplotype. Genes Immun., 2011-05-19;12(7):544-51. 2011-05-19 [PMID: 21593779] (Flow Cytometry, Human)
• Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus. J. Exp. Med., 2010-12-20;208(1):13-21. 2010-12-20 [PMID: 21173105] (Flow Cytometry, Human)
• Beziat V, Descours B, Parizot C, Debre P, Vieillard V NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs. PLoS ONE, 2010-08-06;5(8):e11966. 2010-08-06 [PMID: 20700504] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: KIR2DL3
• Uniprot:
  • P43628()