KIR/CD158 Antibody (180704) [Unconjugated]

PBMC lymphocytes were stained with Rabbit Anti-Human NCAM‑1/CD56 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB24086N) and either (A) Mouse Anti-Human KIR/CD158 Monoclonal Antibody (Catalog # MAB1848) or (B) isotype control antibody (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 180704
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

KIR/CD158 Antibody (180704) [Unconjugated] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human KIR2DL3
• Specificity: Detects human KIR/CD158. Stains cells transfected with KIR2DL2, 2DL3, 2DS2, or 2DS4. It does not stain cells transfected with KIR2DL1, 2DL4, 3DL1, or 3DL2.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIR2DL1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KIR/CD158 Antibody (180704) [Unconjugated]

• CD158 antigen-like family member A
• CD158
• CD158a antigen
• CD158Ankat1
• cl-42
• killer cell immunoglobulin-like receptor 2DL1
• killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1
• killer Ig receptor
• killer inhibitory receptor 2-2-1
• KIR
• KIR221
• MHC class I NK cell receptor
• Natural killer-associated transcript 1
• NKAT
• NKAT-1
• NKAT1KIR-K64
• p58 killer cell inhibitory receptor KIR-K64
• p58 natural killer cell receptor clones CL-42/47.11
• p58 NK cell inhibitory receptor NKR-K6,47.11
• p58 NK receptor CL-42/47.11
• p58.1 MHC class-I-specific NK receptor
• p58.1

Background

KIR is a family of immunoglobulin-like receptors expressed on NK cells and some T cells. Family members with long (L) cytoplasmic tails deliver inhibitory signals and those with short (S) cytoplasmic tails deliver activating signals. Several KIR proteins are known to recognize particular alleles of MHC class I proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KIR/CD158 Antibody (MAB1848)(3)

Publications using MAB1848

• Marin, D;Li, Y;Basar, R;Rafei, H;Daher, M;Dou, J;Mohanty, V;Dede, M;Nieto, Y;Uprety, N;Acharya, S;Liu, E;Wilson, J;Banerjee, P;Macapinlac, HA;Ganesh, C;Thall, PF;Bassett, R;Ammari, M;Rao, S;Cao, K;Shanley, M;Kaplan, M;Hosing, C;Kebriaei, P;Nastoupil, LJ;Flowers, CR;Moseley, SM;Lin, P;Ang, S;Popat, UR;Qazilbash, MH;Champlin, RE;Chen, K;Shpall, EJ;Rezvani, K; Safety, efficacy and determinants of response of allogeneic CD19-specific CAR-NK cells in CD19+ B cell tumors: a phase 1/2 trial Nature medicine 2024-01-18 [PMID: 38238616] (CyTof, Human)
• T Mkorombind, TK Tran-Nguye, K Yuan, Y Zhang, J Xue, GJ Criner, YI Kim, JM Pilewski, A Gaggar, MH Cho, FC Sciurba, SR Duncan HLA-C and KIR permutations influence chronic obstructive pulmonary disease risk JCI Insight, 2021-10-08;6(19):. 2021-10-08 [PMID: 34464355] (Functional Assay, Human)
• Hoglund R, Holmoy T, Harbo H, Maghazachi A A one year follow-up study of natural killer and dendritic cells activities in multiple sclerosis patients receiving glatiramer acetate (GA). PLoS ONE, 2013-04-22;8(4):e62237. 2013-04-22 [PMID: 23614042] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: KIR2DL1