Western Blot: Kinesin 5B Antibody [NB100-1059] - (0.03ug/ml) staining of lysates of cell line HeLa (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Kinesin 5B Antibody [NB100-1059] - (1ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Please note this data was ...read more
Immunohistochemistry-Paraffin: Kinesin 5B Antibody [NB100-1059] - (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Please note this data was ...read more
WB: Approx. 110 kDa band observed in Jurkat and HeLa lysates (calculated MW of 110 kDa band according to NP_004512.1). IHC-P: Human kidney shows perinuclear labelling of some glomerular cells and cytoplasmic staining of renal tubules. Data obtained from a previous batch (different goat). Human uterus shows strong staining of the epithelium on endometrial glands. Data obtained from a previous batch (different goat).
Predicted cross-reactivity based on sequence identity: Mouse, Rat, Bovine.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris (pH 7.3), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Kinesin 5B Antibody
Conventional kinesin heavy chain
kinesin family member 5B
kinesin-1 heavy chain
KNS1kinesin 1 (110-120kD)
Ubiquitous kinesin heavy chain
UKHCkinesin heavy chain
KIF5B is a kinesin. These are microtubule based motor proteins involved in the transport of organelles in eukaryotic cells. They typically consist of 2 identical, approximately 110 to 120 kD heavy chains and 2 identical, approximately 60 to 70 kD light chains. The heavy chain contains 3 domains: a globular N terminal motor domain, which converts the chemical energy of ATP into a motile force along microtubules in 1 fixed direction; a central alpha helical rod domain, which enables the 2 heavy chains to dimerize; and a globular C terminal domain, which interacts with light chains and possibly an organelle receptor.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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