Kinesin 5B Antibody

Western Blot: Kinesin 5B Antibody [NB100-1059] - Staining of HeLa cell lysates (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Kinesin 5B Antibody Summary

• Immunogen: Peptide with sequence C-QPVAVRGGGGKQV corresponding to C-Terminus according to NP_004512.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KIF5B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.5 - 1.5 ug/mL
• Application Notes: WB: Approx. 110 kDa band observed in Jurkat and HeLa lysates (calculated MW of 110 kDa band according to NP_004512.1).
• Control:

  Human Kidney Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Kinesin 5B Antibody

• Conventional kinesin heavy chain
• kinesin family member 5B
• kinesin-1 heavy chain
• KNS1kinesin 1 (110-120kD)
• KNSKINH
• Ubiquitous kinesin heavy chain
• UKHCkinesin heavy chain

Background

KIF5B is a kinesin. These are microtubule based motor proteins involved in the transport of organelles in eukaryotic cells. They typically consist of 2 identical, approximately 110 to 120 kD heavy chains and 2 identical, approximately 60 to 70 kD light chains. The heavy chain contains 3 domains: a globular N terminal motor domain, which converts the chemical energy of ATP into a motile force along microtubules in 1 fixed direction; a central alpha helical rod domain, which enables the 2 heavy chains to dimerize; and a globular C terminal domain, which interacts with light chains and possibly an organelle receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kinesin 5B Antibody (NB100-1059)(3)

Publications using NB100-1059

• AvilEs EC, Krol A, Henle SJ et al. Fat3 acts through independent cytoskeletal effectors to coordinate asymmetric cell behaviors during polarized circuit assembly Cell reports 2022-02-01 [PMID: 35108541] (WB, Mouse)
• Cheng H, Burroughs-Garcia J, Birkness JE et al. Disparate Regulatory Mechanisms Control Fat3 and P75NTR Protein Transport through a Conserved Kif5-Interaction Domain. PLoS ONE. 2016-10-27 [PMID: 27788242] (WB, Human)
• Semiz S, Park JG, Nicoloro SM et al. Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements onmicrotubules EMBO J 2003-05-15 [PMID: 12743033]

Bioinformatics

• Gene Symbol: KIF5B
• Entrez:
  • 3799(Human)
• Uniprot:
  • P33176(Human)