Kinesin 5B Antibody

Images

 
Western Blot: Kinesin 5B Antibody [NB100-1059] - (0.03ug/ml) staining of lysates of cell line HeLa (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Kinesin 5B Antibody [NB100-1059] - (1ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Please note this data was ...read more
Immunohistochemistry-Paraffin: Kinesin 5B Antibody [NB100-1059] - (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Please note this data was ...read more

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Kinesin 5B Antibody Summary

Immunogen
Peptide with sequence C-QPVAVRGGGGKQV corresponding to C-Terminus according to NP_004512.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KIF5B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.5 ug/ml
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2-3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 110 kDa band observed in Jurkat and HeLa lysates (calculated MW of 110 kDa band according to NP_004512.1). IHC-P: Human kidney shows perinuclear labelling of some glomerular cells and cytoplasmic staining of renal tubules. Data obtained from a previous batch (different goat). Human uterus shows strong staining of the epithelium on endometrial glands. Data obtained from a previous batch (different goat).
Control
Kidney Lysate (NB820-59231)
Publications
Read Publications using
NB100-1059 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Kinesin 5B Antibody

  • Conventional kinesin heavy chain
  • kinesin family member 5B
  • kinesin-1 heavy chain
  • KNS1kinesin 1 (110-120kD)
  • KNSKINH
  • Ubiquitous kinesin heavy chain
  • UKHCkinesin heavy chain

Background

KIF5B is a kinesin. These are microtubule based motor proteins involved in the transport of organelles in eukaryotic cells. They typically consist of 2 identical, approximately 110 to 120 kD heavy chains and 2 identical, approximately 60 to 70 kD light chains. The heavy chain contains 3 domains: a globular N terminal motor domain, which converts the chemical energy of ATP into a motile force along microtubules in 1 fixed direction; a central alpha helical rod domain, which enables the 2 heavy chains to dimerize; and a globular C terminal domain, which interacts with light chains and possibly an organelle receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Kinesin 5B Antibody (NB100-1059)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KIF5B
Entrez
Uniprot