Kinesin 5B Antibody (KN-02)

Immunocytochemistry/Immunofluorescence: Kinesin 5B Antibody (KN-02) [NB500-530] - Staining of vesicles (red) in RBL-2H3 rat basophilic leukemia cell line using anti-Kinesin (KN-02). Nuclei were stained with DAPI (blue).

• Reactivity: Hu, Mu, Rt, Po
• Applications: ICC/IF
• Clone: KN-02
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Kinesin 5B Antibody (KN-02) Summary

• Immunogen: Enriched fraction of porcine brain kinesin.
• Localization: Microtubules
• Specificity: The antibody Kinesin 5B recognizes heavy chain of conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. Epitope is located in coiled-coil stalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies KN-01, KN-02 and KN-03 react with different sets of fragments. The antibody KN-02 does not react with kinesin bound to taxol-stabilized microtubules.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIF5B
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS (pH 8.0)
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Multi-step

Alternate Names for Kinesin 5B Antibody (KN-02)

• Conventional kinesin heavy chain
• kinesin family member 5B
• kinesin-1 heavy chain
• KNS1kinesin 1 (110-120kD)
• KNSKINH
• Ubiquitous kinesin heavy chain
• UKHCkinesin heavy chain

Background

Kinesin belongs to the group of microtubule-associated motor proteins known to convert chemical energy released from nucleoside triphosphates (preferentially from ATP) into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport in neuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130 kDa) and two light chains (60 to 70 kDa), resulting in a molecular mass about 400 kDa. Each heavy chain contains an N-terminal globular motordomain with both a microtubule-binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C-terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIF5B
• Entrez:
  • 3799(Human)
  • 16573(Mouse)
  • 117550(Rat)