KIF14 Antibody

Western Blot: KIF14 Antibody [NB100-254] - Detection of Human KIF14 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KIF14 antibody NB100-254 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoprecipitation: KIF14 Antibody [NB100-254] - Detection of human KIF14 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KIF14 antibody NB100-254 (lot 2) used for IP at 6 ug per reaction. KIF14 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-KIF14 antibody NB100-243. For blotting immunoprecipitated KIF14, NB100-254 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

KIF14 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 900 and 950 of human Kinesin Family Member 14 using the numbering given in entry NP_055690.1 (GeneID 9928).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIF14
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:2000-1:10000
• Application Notes: By Western blot, this antibody recognizes bands around 190 kDa representing KIF14.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for KIF14 Antibody

• KIAA0042MGC142302
• kinesin family member 14
• kinesin-like protein KIF14

Background

The kinesin superfamily of proteins consists of over forty KIF motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. KIF14 silencing experiments suggest that KIF14 plays a multi-functional role in mitosis and cytokinesis. KIF14 as also been identified as a candidate oncogene whose overexpression may lead to genomic instability and act as a reporter of poor-prognosis in breast cancer. Alternate identifiers for KIF14 include kinesin family member 14, kinesin-like protein KIF14, KIAA0042, and MGC142302.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KIF14 Antibody (NB100-254)(3)

Publications using NB100-254

• Madhavan J, Coral K, Mallikarjuna K et al. High expression of KIF14 in retinoblastoma: association with older age at diagnosis. Invest Ophthalmol Vis Sci 2007-11-01 [PMID: 17962437]
• Gruneberg U, Neef R, Li X et al. KIF14 and citron kinase act together to promote efficient cytokinesis. J Cell Biol 2006-01-01 [PMID: 16431929]
• Corson TW, Huang A, Tsao MS et al. KIF14 is a candidate oncogene in the 1q minimal region of genomic gain in multiple cancers. Oncogene 2005-07-01 [PMID: 15897902]

Bioinformatics

• Gene Symbol: KIF14
• Entrez:
  • 9928(Human)
• Uniprot:
  • NP_055690.1(Human)