Ki67/MKI67 Antibody

Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB600-1209] - Human Tonsil stained with anti-Ki-67 antibody.

Product Discontinued

Ki67/MKI67 Antibody Summary

• Immunogen: The immunogen for this KI67/MKI67 Antibody was made using a synthetic peptide from the internal region of Human KI67/MKI67, 1200-1300 (C terminal). The exact sequence is proprietary.
• Localization: Nuclear
• Marker: Proliferation Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MKI67
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:300
• Application Notes: For Immunohistochemistry, staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Ki67/MKI67 Antibody was used for ICC/IF (PMID: 23751911) and IHC-Fr (PMID: 18285570).
  Simple Western reported by an internal validation. Separated by Size-All. Apparent MW in kDa on Simple Western was ~400; matrix was 12-180.
• Theoretical MW: 359 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Ki67/MKI67 Antibody reacted with Mouse reported in scientific literature (PMID: 18285570).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris buffered saline, 1% BSA. pH: 7.6
• Preservative: 0.1% Sodium Azide
• Concentration: 0.4 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Ki67/MKI67 Antibody

• antigen identified by monoclonal Ki-67
• Antigen Ki67
• antigen KI-67
• Ki67
• Ki-67
• KIA
• Marker Of Proliferation Ki-67
• MIB-
• MIB-1
• MKI67
• PPP1R105
• Proliferation Marker Protein Ki-67
• proliferation-related Ki-67 antigen
• Protein Phosphatase 1
• Regulatory Subunit 105
• TSG126

Background

Ki67 is a nuclear protein associated with proliferation, named after its discovery in a Hodgkin's lymphoma-derived cell line by Scholzer and Gerdes at Kiel University, Germany (1). This proliferation marker is expressed during active phases of the cell cycle (late G1, S, G2 and M), whereas the protein remains undetectable in the G0 phase. Ki67 has two main isoforms with theoretical molecular weights of 319 kDa and 359 kDa. Its abundance is tightly regulated by synthesis and degradation with an estimated half-life of 60-90 min, regardless of its position in the cell cycle. Ki67 has been shown to be involved in ribosomal biogenesis, heterochromatin maintenance, and mitotic chromosome separation (2).

Detection of Ki67 by immunostaining is commonly used as a proliferation marker in solid tumors, as well as certain hematological malignancies (3-5). The Ki67 index, which reports on positive Ki67 stained tumor cell nuclei, has been extensively studied as a prognostic biomarker in cancers such as breast cancer and cervical cancer.

References

1. Gerdes J, Schwab U, Lemke H, Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer. 31:13-20. PMID: 6339421

2. Starborg M, Gell K, Brundell E and Hoog C. (1996) The murine Ki-67 cell proliferation antigen accumulates in the nucleolar and heterochromatic regions of interphase cells and at the periphery of the mitotic chromosomes in a process essential for cell cycle progression. J Cell Sci. 109:143-153. 1996

3. Karamitopoulou E, Perentes E, Tolnay M, Probst A. (1998) Prognostic significance of MIB-1, p53, and bcl-2 immunoreactivity in meningiomas. Hum Pathol. 29(2):140-5. PMID: 9490273

4. Geyer FC, Rodrigues DN, Weigelt B and Reis-Filho JS. (2012) Molecular classification of estrogen receptor-positive/luminal breast cancers. Adv Anat Pathol. 19(1):39-53. PMID: 22156833

5. Ikenberg H, Bergeron C, Schmidt D, Griesser H, Alameda F, Angeloni C, Bogers J, Dachez R, Denton K, Hariri J, Keller T, von Knebel Doeberitz M, Neumann HH, Puig-Tintore LM, Sideri M, Rehm S, Ridder R; PALMS Study Group. (2013) Screening for cervical cancer precursors with p16/Ki-67 dual-stained cytology: results of the PALMS study. J Natl Cancer Inst. 105(20):1550-7. PMID: 24096620

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ki67/MKI67 Antibody (NB600-1209)(17)

Publications using NB600-1209

• Chen H, Liu J, Shi GP, Zhang X Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice STAR protocols 2023-02-24 [PMID: 36861836] (ICC/IF, Mouse)
• Obayashi N, Sakayori N, Kawaguchi H, Sugita M Effect of irinotecan administration on amiloride-sensitive sodium taste responses in mice European journal of oral sciences 2023-04-01 [PMID: 36852977] (Immunohistochemistry)
• Li C, Li J, Loreno E et al. Protective Effect of Low-Dose Alcohol Consumption against Post-Ischemic Neuronal Apoptosis: Role of L-PGDS International Journal of Molecular Sciences 2021-12-23 [PMID: 35008575]
• Kozuki S, Sakurai S, Suzuki A et al. Delineation of biliary epithelial cell dynamics in maternal liver during pregnancy Genes to cells : devoted to molecular & cellular mechanisms 2021-12-30 [PMID: 34967957]
• Zalles M, Smith N, Saunders D et al. A tale of two multi-focal therapies for glioblastoma: An antibody targeting ELTD1 and nitrone-based OKN-007 Journal of cellular and molecular medicine 2021-12-14 [PMID: 34910361] (IHC-P, Mouse)
• Hu Y, Zhu Y, Gerber SD et al. Deletion of Nrip1 delays skin aging by reducing adipose-derived mesenchymal stem cells (ADMSCs) senescence, and maintaining ADMSCs quiescence GeroScience 2021-03-11 [PMID: 33704619]
• Ichijo R, Kabata M, Kidoya H et al. Vasculature-driven stem cell population coordinates tissue scaling in dynamic organs Science advances 2021-02-01 [PMID: 33568475] (IHC-Fr, Mouse)
• Zhang Y, Zhu H, Layritz F et Al. Recombinant adenosine deaminase ameliorates inflammation, vascular disease and fibrosis in preclinical models of systemic sclerosis Arthritis & rheumatology (Hoboken, N.J.) 2020-03-17 [PMID: 32182396]
• Zhang X, Wang X, Yin H et al. Functional Inactivation of Mast Cells Enhances Subcutaneous Adipose Tissue Browning in Mice Cell Rep 2019-07-16 [PMID: 31315055] (IHC-P, Mouse)
• Ichijo R, Kobayashi H, Yoneda S et al. Tbx3-dependent amplifying stem cell progeny drives interfollicular epidermal expansion during pregnancy and regeneration Nat Commun 2017-09-11 [PMID: 28894084] (IHC-Fr, Mouse)
• Rizvi S, Yamada D, Hirsova P et al. A Hippo and Fibroblast Growth Factor Receptor Autocrine Pathway in Cholangiocarcinoma J Biol Chem. 2016-01-29 [PMID: 26826125] (IHC-Fr, Human)
• Suzuki D, Sahu R, Leu NA, Senoo M. The carboxy-terminus of p63 links cell cycle control and the proliferative potential of epidermal progenitor cells. Development. 2015-01-15 [PMID: 25503409] (Mouse)
• Chakraborty S, Sengupta A, Yutzey KE. Tbx20 promotes cardiomyocyte proliferation and persistence of fetal characteristics in adult mouse hearts. J Mol Cell Cardiol 2013-06-07 [PMID: 23751911] (ICC/IF, IHC-P, Mouse)
• Song Y, Santen RJ, Wang JP, Yue W. Effects of the Conjugated Equine Estrogen/Bazedoxifene Tissue-Selective Estrogen Complex (TSEC) on Mammary Gland and Breast Cancer in Mice Endocrinology 2012-12-01 [PMID: 23070546] (IF/IHC, Mouse)
• Zhao Yong, Lin Brian, Darflinger Robert et al. Human cord blood stem cell-modulated regulatory T lymphocytes reverse the autoimmune-caused type 1 diabetes in nonobese diabetic (NOD) mice. PLoS One 2009-01-01 [PMID: 19156219] (IF/IHC, Mouse)
• Towner R, Smith N, Saunders D et al. Targeting Bioinformatics Predicted Biomarkers Associated with Cell Proliferation and Migration for Treating Gliomas: Preclinical Studies in a GL261 Mouse Model Neuroglia 2023-03-15 (Immunohistochemistry-Paraffin)
• Olofsson, P S, L A Soderstrom et al. CD137 Is Expressed in Human Atherosclerosis Promotes Development of Plaque Inflammation in Hypercholesterolemic Mice. Circulation: CIRCULATIONAHA107.699173. 2008-01-01 [PMID: 18285570]

Review for Ki67/MKI67 Antibody (NB600-1209) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 07/20/2015

Summary

• Application: Immunofluorescence
• Sample Tested: -
• Species: Mouse

FAQs for Ki67/MKI67 Antibody (NB600-1209). (Showing 1 - 1 of 1 FAQs).

1. Is there a clone number for this Ki67 antibody?
   • This antibody is polyclonal, and thus it does not have a clone number.

Bioinformatics

• Gene Symbol: MKI67
• Entrez:
  • 4288(Human)
  • 17345(Mouse)
• Uniprot:
  • P46013(Human)