Ki-67/MKI67 Antibody Summary
Synthetic peptide from C-terminus of Human Ki67.(Note: the amino acid sequence is proprietary).
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Immunocytochemistry/Immunofluorescence 1:10-1:500 Immunohistochemistry 1:10-1:500 Immunohistochemistry-Frozen Immunohistochemistry-Paraffin 1:300
IHC: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Use in ICC/IF was reported in the scientific literature (PMID: 23751911). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 18285570)
Mouse reactivity reported in scientific literature (PMID: 18285570).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS (pH 7.6) and 1.0% BSA
0.1% Sodium Azide
Immunogen affinity purified
Alternate Names for Ki-67/MKI67 Antibody
antigen identified by monoclonal Ki-67 antigen KI-67 Ki67 Ki-67 KIA Marker Of Proliferation Ki-67 MIB-1 MKI67 PPP1R105 proliferation-related Ki-67 antigen Protein Phosphatase 1 Regulatory Subunit 105 TSG126 Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
for 1 year from date of receipt.
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Applications: WB, ICC/IF, IHC, IHC-P, IP
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Applications: ICC/IF, IHC, IHC-P
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Species: Hu, Rt
Applications: WB, ELISA
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
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Publications for Ki-67/MKI67 Antibody (NB600-1209)(6)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 5 applications: ICC/IF, IHC, IHC-FR, IHC-Fr, IHC-P.
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Showing Publications 1 -
Publications using NB600-1209
Ichijo R, Kobayashi H, Yoneda S et al. Tbx3-dependent amplifying stem cell progeny drives interfollicular epidermal expansion during pregnancy and regeneration Nat Commun 2017 Sep 11 [PMID: 28894084] (IHC-FR, Mouse) IHC-FR
Rizvi S, Yamada D, Hirsova P et al. A Hippo and Fibroblast Growth Factor Receptor Autocrine Pathway in Cholangiocarcinoma J. Biol. Chem. Jan 29 2016 [PMID: 26826125] (IHC-Fr, Human) IHC-Fr
Suzuki D, Sahu R, Leu NA, Senoo M. The carboxy-terminus of p63 links cell cycle control and the proliferative potential of epidermal progenitor cells. Development. 2015 Jan 15 [PMID: 25503409] (Mouse)
Chakraborty S, Sengupta A, Yutzey KE. Tbx20 promotes cardiomyocyte proliferation and persistence of fetal characteristics in adult mouse hearts. J Mol Cell Cardiol 2013 Jun 7 [PMID: 23751911] (IHC-P, ICC/IF, Mouse) IHC-P, ICC/IF
Song Y, Santen RJ, Wang JP, Yue W. Effects of the Conjugated Equine Estrogen/Bazedoxifene Tissue-Selective Estrogen Complex (TSEC) on Mammary Gland and Breast Cancer in Mice Endocrinology 2012 Dec [PMID: 23070546] (IHC, Mouse) IHC
Olofsson PS, Soderstrom LA, Wagsater D et al. CD137 Is Expressed in Human Atherosclerosis Promotes Development of Plaque Inflammation in Hypercholesterolemic Mice. Circulation;117(10):1292-301. March 11 2008. [PMID: 18285570]
Ki-67/MKI67 Antibody (NB600-1209) (1)
Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
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Reviews using NB600-1209:
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Application Immunofluorescence Sample Tested - Species Mouse Blocking
Blocking Details M.O.M blocking solution (1 hour at RT) Primary Anitbody
Dilution Ratio 1:100 for 2 hours at RT Secondary Antibody
Secondary Description anti-rabbit Details
Detection Notes fluorescent microscope
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FAQs for Ki-67/MKI67 Antibody (NB600-1209) (0)
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Blogs on Ki-67/MKI67.
The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life. First discovered to be located to dividing cells,... Read full blog post.
Ki67 - an established marker for labelling proliferating cells
Ki-67/MKI67 is an antigen which is expressed during G1, S, G2, and M phases of the cell cycle (mitotically active cells), but not during G0 phase (resting cells). It is a large protein with expected molecular weight of about 395 kDa, and it has a v... Read full blog post.
Ki67 - A Crucial Cellular Proliferation Marker
The Ki67 antigen is a prototypic cell cycle-related protein expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M). It is a non-histone nuclear protein originally identified in a Hodgkin's lymphoma-derived cell line.... Read full blog post.
The Ki67 Antibody in Cell Marker Studies
The MK167, or Ki67 antibody recognizes a nuclear protein encoded by the MK167 gene. Ki167 is involved with RNA transcription and essential to cellular proliferation, being expressed by proliferating cells at all stages of the active cell cycle; it is... Read full blog post.