Ki-67/MKI67 Antibody

Images

 
Immunohistochemistry-Paraffin: Ki-67/MKI67 Antibody [NB600-1209] - Human Tonsil stained with anti-Ki-67 antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.4 mg/ml

Order Details

Ki-67/MKI67 Antibody Summary

Immunogen
Synthetic peptide from C-terminus of Human Ki67.(Note: the amino acid sequence is proprietary).
Localization
Nuclear
Marker
Proliferation Marker
Specificity
Ki67
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MKI67
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:300
Application Notes
IHC: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Use in ICC/IF was reported in the scientific literature (PMID: 23751911). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 18285570)
Reviewed Applications
Read 1 Review rated 4
using
NB600-1209 in the following applications:

Publications
Read Publications using
NB600-1209 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 18285570).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.6) and 1.0% BSA
Preservative
0.1% Sodium Azide
Concentration
0.4 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ki-67/MKI67 Antibody

  • antigen identified by monoclonal Ki-67
  • antigen KI-67
  • Ki67
  • Ki-67
  • KIA
  • Marker Of Proliferation Ki-67
  • MIB-1
  • MKI67
  • PPP1R105
  • proliferation-related Ki-67 antigen
  • Protein Phosphatase 1
  • Regulatory Subunit 105
  • TSG126

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ki-67/MKI67 Antibody (NB600-1209)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-FR, IHC-Fr, IHC-P.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-FR
(1)
IHC-Fr
(1)
IHC-P
(1)
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Human
(1)
Mouse
(4)
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Review for Ki-67/MKI67 Antibody (NB600-1209) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IF.

Reviews using NB600-1209:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Ki-67/MKI67 NB600-1209
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reviewed by:
Anonymous
IF Mouse 07/20/2015
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Summary

ApplicationImmunofluorescence
Sample Tested-
SpeciesMouse

Blocking

Blocking DetailsM.O.M blocking solution (1 hour at RT)

Primary Anitbody

Dilution Ratio1:100 for 2 hours at RT

Secondary Antibody

Secondary Descriptionanti-rabbit

Details

Detection Notesfluorescent microscope

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Ki-67/MKI67 Antibody (NB600-1209) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Ki-67/MKI67 Antibody (NB600-1209)

Discover related pathways, diseases and genes to Ki-67/MKI67 Antibody (NB600-1209). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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PTMs for Ki-67/MKI67 Antibody (NB600-1209)

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Research Areas for Ki-67/MKI67 Antibody (NB600-1209)

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Blogs on Ki-67/MKI67.

The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

Ki67 - an established marker for labelling proliferating cells
Ki-67/MKI67 is an antigen which is expressed during G1, S, G2, and M phases of the cell cycle (mitotically active cells), but not during G0 phase (resting cells). It is a large protein with expected molecular weight of about 395 kDa, and it has a v...  Read full blog post.

Ki67 - A Crucial Cellular Proliferation Marker
The Ki67 antigen is a prototypic cell cycle-related protein expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M). It is a non-histone nuclear protein originally identified in a Hodgkin's lymphoma-derived cell line....  Read full blog post.

The Ki67 Antibody in Cell Marker Studies
The MK167, or Ki67 antibody recognizes a nuclear protein encoded by the MK167 gene. Ki167 is involved with RNA transcription and essential to cellular proliferation, being expressed by proliferating cells at all stages of the active cell cycle; it is...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
07/20/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol MKI67
Entrez
Uniprot