KGF/FGF-7 Antibody (299026) [Alexa Fluor® 594]

• Reactivity: Ca
• Applications: IHC, ICC/IF
• Clone: 299026
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

KGF/FGF-7 Antibody (299026) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant canine KGF/FGF‑7
  Cys32-Thr194
  Accession # P79150
• Specificity: Detects canine KGF/FGF‑7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) FGF-11, -12, -13, -16, -20,
  -21, -22, -23, or rhFGFbasic is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KGF/FGF-7 Antibody (299026) [Alexa Fluor® 594]

• FGF7
• FGF-7
• fibroblast growth factor 7HBGF-7
• HBGF7
• HBGF-7
• Heparin-binding growth factor 7
• keratinocyte growth factor
• KGF
• KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

The fibroblast growth factor (FGF) family consists of twenty three known structurally-related proteins that play key roles in development, morphogenesis, angiogenesis, wound healing and tumorigenesis (1). All FGFs share a common structural fold, the beta -trefoil scaffold, in a conserved central region of approximately 120 amino acid (aa), which contains the active site and a heparin binding site (2). FGF-7, also known as keratinocyte growth factor (KGF), was originally identified from the conditioned medium of a human embryonic lung fibroblast cell line (3). It is secreted by cells of mesenchymal origin and functions predominantly on epithelial cells (1). The mature caFGF-7 is generated from a 194 aa precursor protein by cleavage of the first 31 aa at the N-terminus. It shares 97.4% and 95.9% sequence identity with human and mouse KGF, respectively (4). FGFs signal through high affinity tyrosine kinase receptors (FGF R) (5). Cellular responses to FGFs are modulated by heparan sulfate proteoglycans that are also known as low affinity FGF receptors and by heparin (6). Various isoforms of the four FGF R exist (FGF R‑1 through -4). They are produced from alternatively spliced transcripts in both the extracellular and intracellular domains. FGF-7 signals through the FGF R2IIIb (also known as KGF R), which is a spliced variant of FGF R2 (7). KGF R binds FGF-1 and FGF-7 with high affinity and FGF-2 with a 20-fold lower affinity (8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.