Ketohexokinase Overexpression Lysate

Western Blot: ketohexokinase Overexpression Lysate (Adult Normal) [NBL1-12232] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ketohexokinase.

• Reactivity: Hu
• Applications: WB

Ketohexokinase Overexpression Lysate Summary

• Description: Transient overexpression lysate of ketohexokinase (fructokinase) (KHK), transcript variant a The lysate was created in HEK293T cells, using Plasmid ID RC202424 and based on accession number NM_000221. The protein contains a C-MYC/DDK Tag.
• Gene: KHK

Applications/Dilutions

• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 32.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Ketohexokinase Overexpression Lysate

• EC 2.7.1.3
• Fructokinase
• Hepatic fructokinase
• ketohexokinase (fructokinase)
• Ketohexokinase
• KHK

Background

This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]. Transcript Variant: This variant utilizes the alternate exon 3a and encodes isoform a, which exhibits lower enzymatic activity than isoform b.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: KHK