Ketohexokinase Overexpression Lysate

Images

 
Western Blot: ketohexokinase Overexpression Lysate (Adult Normal) [NBL1-12232] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ketohexokinase.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Ketohexokinase Overexpression Lysate Summary

Description
Transient overexpression lysate of ketohexokinase (fructokinase) (KHK), transcript variant a The lysate was created in HEK293T cells, using Plasmid ID RC202424 and based on accession number NM_000221. The protein contains a C-MYC/DDK Tag.
Gene
KHK

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
32.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Ketohexokinase Overexpression Lysate

  • EC 2.7.1.3
  • Fructokinase
  • Hepatic fructokinase
  • ketohexokinase (fructokinase)
  • Ketohexokinase
  • KHK

Background

This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]. Transcript Variant: This variant utilizes the alternate exon 3a and encodes isoform a, which exhibits lower enzymatic activity than isoform b.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-47980
Species: Hu, Pm
Applications: WB, Flow, IHC, IHC-P, IP
NBP2-70822
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-236
Species: Hu, Mu
Applications: WB, IB, IHC, IHC-Fr, IP
NBP1-84127
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-75717
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-87416
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-32317
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-02164
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-67560
Species: Hu, Mu, Rt, Ze
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-03396
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-03621
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-57033
Species: Hu
Applications: WB
NBP2-75418
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-90233
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-92824
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-70092
Species: Hu, Rt, Ca
Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
NBP2-75398
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-62658
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-67656
Species: Hu, Mu, Rt, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP

Publications for Ketohexokinase Lysate (NBL1-12232) (0)

There are no publications for Ketohexokinase Lysate (NBL1-12232).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Ketohexokinase Lysate (NBL1-12232) (0)

There are no reviews for Ketohexokinase Lysate (NBL1-12232). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Ketohexokinase Lysate (NBL1-12232) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-12232

Additional Ketohexokinase Products

Bioinformatics Tool for Ketohexokinase Lysate (NBL1-12232)

Discover related pathways, diseases and genes to Ketohexokinase Lysate (NBL1-12232). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Ketohexokinase Lysate (NBL1-12232)

Discover more about diseases related to Ketohexokinase Lysate (NBL1-12232).
 

Pathways for Ketohexokinase Lysate (NBL1-12232)

View related products by pathway.

PTMs for Ketohexokinase Lysate (NBL1-12232)

Learn more about PTMs related to Ketohexokinase Lysate (NBL1-12232).
 

Research Areas for Ketohexokinase Lysate (NBL1-12232)

Find related products by research area.

Blogs on Ketohexokinase

There are no specific blogs for Ketohexokinase, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Ketohexokinase Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol KHK