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We offer Ketohexokinase Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each Ketohexokinase Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Ketohexokinase Antibodies can be used in a variety of model species: Human, Monkey, Mouse. Use the list below to choose the Ketohexokinase Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Ketohexokinase polyclonal antibodies and browse our Ketohexokinase monoclonal antibody catalog.
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-85790] - Staining in human small intestine and skeletal muscle tissues . Corresponding KHK RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-85790-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-85790] - Staining in human small intestine and skeletal muscle tissues . Corresponding KHK RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-85790] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-85790-img0014.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-85790] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-85790-img0015.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-85790] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-85790-img0016.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-85790] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-85790-img0017.jpg)
![Western Blot: ketohexokinase Antibody (1D8) [NBP2-02639] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ketohexokinase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ketohexokinase.](http://images.novusbio.com/fullsize/ketohexokinase-Antibody-1D8-Western-Blot-NBP2-02639-img0002.jpg "Western Blot: ketohexokinase Antibody (1D8) [NBP2-02639] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ketohexokinase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ketohexokinase.")
![Immunocytochemistry/Immunofluorescence: Ketohexokinase Antibody (OTI1D8) [NBP2-02639] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ketohexokinase.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1D8-Immunocytochemistry-Immunofluorescence-NBP2-02639-img0010.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody (1D8) [NBP2-02639] - ketohexokinase Antibody (1D8) [NBP2-02639] - Staining of paraffin-embedded Human thyroid tissue using anti-ketohexokinase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1D8-Immunohistochemistry-Paraffin-NBP2-02639-img0015.jpg)
![Flow Cytometry: Ketohexokinase Antibody (1D8) [NBP2-02639] - ketohexokinase Antibody (1D8) [NBP2-02639] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ketohexokinase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1D8-Flow-Cytometry-NBP2-02639-img0009.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody (1D8) [NBP2-02639] - ketohexokinase Antibody (1D8) [NBP2-02639] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ketohexokinase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1D8-Immunohistochemistry-Paraffin-NBP2-02639-img0011.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody (1D8) [NBP2-02639] - ketohexokinase Antibody (1D8) [NBP2-02639] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ketohexokinase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1D8-Immunohistochemistry-Paraffin-NBP2-02639-img0012.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody (1D8) [NBP2-02639] - ketohexokinase Antibody (1D8) [NBP2-02639] - Staining of paraffin-embedded Human Kidney tissue using anti-ketohexokinase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1D8-Immunohistochemistry-Paraffin-NBP2-02639-img0013.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody (1D8) [NBP2-02639] - ketohexokinase Antibody (1D8) [NBP2-02639] - Staining of paraffin-embedded Human liver tissue using anti-ketohexokinase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1D8-Immunohistochemistry-Paraffin-NBP2-02639-img0014.jpg)
![Western Blot: Ketohexokinase Antibody [NBP1-32778] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ketohexokinase antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Western-Blot-NBP1-32778-img0009.jpg "Western Blot: Ketohexokinase Antibody [NBP1-32778] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ketohexokinase antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: ketohexokinase Antibody [NBP1-32778] - Sample (20 ug of whole cell lysate) A: mouse Liver 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/ketohexokinase-Antibody-Western-Blot-NBP1-32778-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Ketohexokinase Antibody [NBP1-32778] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ketohexokinase antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Western-Blot-NBP1-32778-img0007.jpg)
![Immunohistochemistry-Paraffin: ketohexokinase Antibody [NBP1-32778] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ketohexokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-32778-img0002.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-32778] - Paraffin-embedded mouse kidney. Ketohexokinase antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-Immunohistochemistry-Paraffin-NBP1-32778-img0005.jpg)
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody [NBP1-32778] - Ketohexokinase antibody [N1C3] detects Ketohexokinase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Ketohexokinase stained by Ketohexokinase antibody [N1C3] (NBP1-32778) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-32778_rabbit-polyclonal-ketohexokinase-antibody-91020242021965.jpg)
![Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Analysis of KHK expression in transfected 293T cell line by KHK monoclonal antibody (M01), clone 1H6-2B6.Lane 1: KHK transfected lysate(32.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1H6-2B6-Western-Blot-H00003795-M01-img0019.jpg "Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Analysis of KHK expression in transfected 293T cell line by KHK monoclonal antibody (M01), clone 1H6-2B6.Lane 1: KHK transfected lysate(32.7 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Analysis of monoclonal antibody to KHK on formalin-fixed paraffin-embedded human spleen tissue. Antibody concentration 1 ~ 10 ug/ml.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1H6-2B6-Immunohistochemistry-Paraffin-H00003795-M01-img0015.jpg)
![Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - KHK expression in human spleen.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1H6-2B6-Western-Blot-H00003795-M01-img0012.jpg)
![Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Analysis of KHK over-expressed 293 cell line, cotransfected with KHK Validated Chimera RNAi ( Cat # H00003795-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KHK monoclonal antibody (M01), clone 1H6-2B6 (Cat # H00003795-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1H6-2B6-Western-Blot-H00003795-M01-img0016.jpg)
![Western Blot: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - KHK monoclonal antibody (M01), clone 1H6-2B6. Analysis of KHK expression in human liver.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1H6-2B6-Western-Blot-H00003795-M01-img0018.jpg)
![Sandwich ELISA: Ketohexokinase Antibody (1H6-2B6) [H00003795-M01] - Detection limit for recombinant GST tagged KHK is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-1H6-2B6-Sandwich-ELISA-H00003795-M01-img0017.jpg)
![Immunohistochemistry: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32496_rabbit-ketohexokinase-mab-psh03-18-29820241420952.jpg "Immunohistochemistry: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32496) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Immunocytochemistry analysis of HepG2 cells labeling Ketohexokinase with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32496_rabbit-ketohexokinase-mab-psh03-18-2920241192372.jpg)
![Flow Cytometry: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Flow cytometric analysis of HepG2 cells labeling Ketohexokinase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32496, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32496_rabbit-ketohexokinase-mab-psh03-18-39202488461.jpg)
![Western Blot: Ketohexokinase Antibody (PSH03-18) [NBP3-32496] - Western blot analysis of Ketohexokinase on different lysates with Rabbit anti-Ketohexokinase antibody (NBP3-32496) at 1/2,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Human kidney tissue lysate Lane 3: Mouse liver tissue lysate Lane 4: Mouse kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32496_rabbit-ketohexokinase-mab-psh03-18-392024845350.jpg)
![Western Blot: Ketohexokinase Antibody (PSH03-19) [NBP3-32497] - Western blot analysis of Ketohexokinase on different lysates with Rabbit anti-Ketohexokinase antibody (NBP3-32497) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Human liver tissue lysate Lane 3: Human kidney tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 11 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32497) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32497_rabbit-ketohexokinase-mab-psh03-19-278202416331269.jpg "Western Blot: Ketohexokinase Antibody (PSH03-19) [NBP3-32497] - Western blot analysis of Ketohexokinase on different lysates with Rabbit anti-Ketohexokinase antibody (NBP3-32497) at 1/2,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Human liver tissue lysate Lane 3: Human kidney tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 11 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32497) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Ketohexokinase Antibody (PSH03-19) [NBP3-32497] - Immunocytochemistry analysis of HepG2 cells labeling Ketohexokinase with Rabbit anti-Ketohexokinase antibody (NBP3-32497) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ketohexokinase antibody (NBP3-32497) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32497_rabbit-ketohexokinase-mab-psh03-19-29202410234255.jpg)
![Flow Cytometry: Ketohexokinase Antibody (PSH03-19) [NBP3-32497] - Flow cytometric analysis of HepG2 cells labeling Ketohexokinase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32497, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32497_rabbit-ketohexokinase-mab-psh03-19-29202416453288.jpg)
![Western Blot: Ketohexokinase Antibody (S1) [H00003795-M03] - Analysis of KHK expression in Jurkat (Cat # L017V1).](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-S1-Western-Blot-H00003795-M03-img0004.jpg "Western Blot: Ketohexokinase Antibody (S1) [H00003795-M03] - Analysis of KHK expression in Jurkat (Cat # L017V1).")
![Immunocytochemistry/Immunofluorescence: Ketohexokinase Antibody (S1) [H00003795-M03] - Analysis of monoclonal antibody to KHK on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-S1-Immunocytochemistry-Immunofluorescence-H00003795-M03-img0003.jpg)
![Western Blot: Ketohexokinase Antibody (2B1) [H00003795-M07] - Analysis of KHK expression in Jurkat (Cat # L017V1).](http://images.novusbio.com/fullsize/Ketohexokinase-Antibody-2B1-Western-Blot-H00003795-M07-img0002.jpg "Western Blot: Ketohexokinase Antibody (2B1) [H00003795-M07] - Analysis of KHK expression in Jurkat (Cat # L017V1).")