Keap1 Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line . PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Keap1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3024) followed by HRP-conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Keap1 at approximately 66 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Western blot shows lysates of Jurkat human acute T cell leukemia parental cell line and Keap1 Jurkat cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Keap1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3024) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Keap1 at approximately 66 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in knockout Jurkat cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Keap1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Keap1
  Ala90-Ile250
  Accession # Q14145
• Specificity: Detects endogenous human, mouse and rat Keap1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KEAP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Keap1 Antibody [Unconjugated]

• INrf2
• INrf2MGC9454
• Keap1
• kelch-like ECH-associated protein 1
• Kelch-like protein 19
• KIAA0132MGC4407
• KLHL19Cytosolic inhibitor of Nrf2
• MGC10630
• MGC1114
• MGC20887

Background

Kelch-like ECH-associated protein 1 (Keap1) is a 624 amino acid, 69 kDa protein which interacts with the transcription factor NF-E2-related factor 2 (Nrf2). Keap1 represses Nrf2 function by sequestering Nrf2 in the cytoplasm. Keap1 contains an N-terminal BTB domain and six C-terminal KELCH domains (aa 327‑611) that interact with Nrf2. Dissociation of the two proteins in response to redox-sensitive cell stress is followed by the translocation of Nrf2 to the nucleus and transcription of detoxifying/oxidative stress enzyme genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Keap1 Antibody (AF3024)(1)

Publication using AF3024

• Yamashita , Yasunobu, Ueyama , Takashi, Nishi , Toshio, Yamamoto , Yuta, Kawakoshi , Akatsuki, Sunami , Shogo, Iguchi , Mikitaka, Tamai , Hideyuki, Ueda , Kazuki, Ito , Takao, Tsuruo , Yoshihir, Ichinose , Masao Nrf2-inducing anti-oxidation stress response in the rat liver--new beneficial effect of lansoprazole. PLoS ONE, 2014-05-20;9(5):e97419. 2014-05-20 [PMID: 24846271] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: KEAP1
• Uniprot:
  • Q14145()