Keap1 Antibody (OTI1B4) - Azide and BSA Free

Western Blot: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 (1:1000).

Immunocytochemistry/Immunofluorescence: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - Staining of 293T cells transfected by pCMV6-ENTRY KEAP1 using anti-KEAP1 antibody (green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).

Immunohistochemistry: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - Staining of paraffin-embedded Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.

Flow Cytometry: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - The levels of Keap1 were analyzed and quantified using geometric mean fluorescence intensity (MFI) by flow cytometry. Analysis using PE conjugated version on antibody (NBP2-71496PE). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32765513/) licensed under a CC-BY license.

Western Blot: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues used at a 1:200 dilution.

Western Blot: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - Analysis of extracts (35ug) from 9 different cell lines by using anti-KEAP1 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Western Blot: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - Analysis of human BEAS2B cells.

Western Blot: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells and KEAP1-Knockout Hela cells were separated by SDS-PAGE and immunoblotted with anti-KEAP1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-beta

Immunohistochemistry: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KEAP1 mouse monoclonal antibody.

Immunohistochemistry: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KEAP1 mouse monoclonal antibody.

Flow Cytometry: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - Analysis of Hela cells, using anti-KEAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - Analysis of Jurkat cells, using anti-KEAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

CyTOF-ready: Keap1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-71496] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-KEAP1 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready, KO
• Clone: OTI1B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Keap1 Antibody (OTI1B4) - Azide and BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse Keap1 Antibody (OTI1B4) - Azide and BSA Free (NBP2-03319) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinnt protein of human KEAP1(NP_987096) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KEAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Knockout Validated
  • Western Blot 1:500-2000
• Theoretical MW: 69.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Keap1 Antibody (OTI1B4) - Azide and BSA Free

• INrf2
• INrf2MGC9454
• Keap1
• kelch-like ECH-associated protein 1
• Kelch-like protein 19
• KIAA0132MGC4407
• KLHL19Cytosolic inhibitor of Nrf2
• MGC10630
• MGC1114
• MGC20887

Background

The KEAP1 gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associatedprotein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins inthe cytoplasm is followed

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KEAP1