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KDM6A Antibody (1612)

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Western Blot: KDM6A Antibody (1612) [NBP2-43665] - Analysis of A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 5 % SDS-PAGE KDM6A antibody [1612] dilution: 1:500.
Western Blot: KDM6A Antibody (1612) [NBP2-43665] - Analysis of A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB
Clone
1612
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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KDM6A Antibody (1612) Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human KDM6A. The exact sequence is proprietary.
Predicted Species
Mouse (93%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KDM6A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000

Reactivity Notes

Xenopus laevis (82%), Dog (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for KDM6A Antibody (1612)

  • bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeatprotein (UTX))
  • bA386N14.2
  • DKFZp686A03225
  • EC 1.14.11
  • EC 1.14.11.-
  • Histone demethylase UTX
  • KABUK2
  • KDM6A
  • Lysine (K)specific Demethylase 6A
  • Lysine (K)-specific Demethylase 6A
  • MGC141941
  • ubiquitously transcribed tetratricopeptide repeat protein X-linked
  • ubiquitously transcribed tetratricopeptide repeat, X chromosome
  • ubiquitously transcribed TPR protein on the X chromosome
  • ubiquitously transcribed X chromosome tetratricopeptide repeat protein
  • ubiquitously-transcribed TPR gene on the X chromosome
  • Ubiquitously-transcribed TPR protein on the X chromosome
  • Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein
  • UTX
  • UTXlysine-specific demethylase 6A

Background

UTX - ubiquitously transcribed tetratricopeptide repeat, X chromosome

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-JMJD3 [NBP1-06640] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - KDM6A protein levels are decreased following EMT and upregulated during MET. MCF10A cells, treated as described, were analyzed by immunoblotting for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 and vimentin (Vim). COX IV was used as a loading control. Image collected and cropped by CiteAb from the following publication (http://www.oncotarget.com/fulltext/19214), licensed under a CC-BY licence.
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Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Representative Western blot showing M-track protein protein proximity signals obtained for Kic1. Hog1-protA-H3: background control, Nup2: positive control. Below: Proximity signals. n = 3 replicates per sample except when indicated differently. Ratios are log2-transformed. Black lines indicate average proximity signal. Proximity signals that differ significantly from background are marked in green (q 0.01) filled circles. Grey filled triangles: q > 0.05. N: N-terminal HKMTmyc fusion. Image collected and cropped by CiteAb from the following publication (https://biosignaling.biomedcentral.com/articles/10.1186/s12964-019-0381-z), licensed under a CC-BY licence.
Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Analysis of Histone H3 K9-me3 in HeLa histone lysates. Observed molecular weight is ~15 kDa.
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Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-81657] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MBD2 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656.
(3 Publications)
Species: Hu, Mu
Applications: ChIP, IHC, IHC-P, IP, WB
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Immunohistochemistry-Paraffin: EZH2/KMT6 Antibody (CL11915) [NBP3-15125] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: EZH2/KMT6 Antibody (CL11915) [NBP3-15125] - Staining of human glioblastoma multiforme shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: EZH2/KMT6 Antibody (CL11915) [NBP3-15125] - Staining of human kidney shows mainly absence of positivity in cells in tubules as expected.
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Applications: ICC/IF, IHC, IHC-P, WB
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Immunocytochemistry/Immunofluorescence: KMT2D Antibody [NBP1-89123] - Staining of human cell line A-431 shows localization to nucleus, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: KMT2D Antibody [NBP1-89123] - Staining of human cervix, uterine shows weak nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: KMT2D Antibody [NBP1-89123] - Staining of human rectum shows moderate nuclear positivity in glandular cells.
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Immunohistochemistry-Paraffin: KMT2B Antibody [NB300-864] - (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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(413104) [Unconjugate
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Jak1 was detected in immersion fixed paraffin-embedded sections of human epidermis using 25 µg/mL Rat Anti-Human/Mouse/Rat Jak1 Monoclonal Antibody (Catalog # MAB4260) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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DNMT3A Antibody
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Western Blot: DNMT3A Antibody (64B1446) [NB120-13888] - NTERA-2 cells were treated with Zebularine as indicated for 24 hours. Cell lysates were prepared and separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was probed with 2 ug/mL anti-Dnmt3a in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the decrease in Dnmt3a expression upon treatment with 500 uM Zebularine (arrowhead). Additional bands at 90 and 75 kDa can also be detected with this antibody and may represent alternative splice variants. Tubulin is shown as a loading control.
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Flow Cytometry: DNMT3A Antibody (64B1446) [NB120-13888] - An intracellular stain was performed on HepG2 cells with DNMT3A (64B1446) NB120-13888AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
(3 Reviews)
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Von Hippel Lindau
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Immunohistochemistry: Von Hippel Lindau Antibody (1E1) [NBP2-45411] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Von Hippel Lindau Antibody (1E1) [NBP2-45411] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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(3B9E3) - BSA Free
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Western Blot: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of DDX3X, DDX5 protein expression levels by Western blot in DTMUV-infected and control baby hamster kidney cell line BHK-21. From a verified customer review.
Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of Hela cells using DDX3X mouse mAb (green) and negative control (red).
Flow Cytometry: DDX3 Antibody (3B9E3) [NBP2-52457] - Analysis of HepG2 cells using DDX3X mouse mAb (green) and negative control (red).
(2 Reviews)
(1 Publication)
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Applications: CyTOF-ready, ELISA, Flow, ICC, ICC/IF, IF, IHC, IHC-P, WB
Isocitrate
Dehydrogenase 1/IDH1 ...
NBP1-47804
Knockout Validated: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and IDH1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:2,000 of Mouse Anti-Human IDH1 monoclonal Antibody (Catalog # NBP1-47804) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for IHD1 at approximately 38 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI2H9) [NBP1-47804] - Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Isocitrate dehydrogenase.
Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (2H9) [NBP1-47804] - Staining of paraffin-embedded breast tissue using anti-Isocitrate dehydrogenase mouse monoclonal antibody.
(1 Review)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
KDM6A Antibody
(1612)
NBP2-43665
KDM6A Antibody (1612)
Species: Hu, Mu, Bv
Applications: WB

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Bioinformatics

Gene Symbol KDM6A