KDELR1 Antibody (KR-10) - BSA Free

Western Blot: KDELR1 Antibody (KR-10) [NBP2-12873] - analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: KDELR1 Antibody (KR-10) [NBP2-12873] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDELR1 Monoclonal Antibody, Clone KR-10 (NBP2-12873). Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDELR1 Monoclonal Antibody (NBP2-12873) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images. Courtesy of: Institute of Mol. and Cell Bio, Singapore.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Ha, Ma, Pm, Rb, Sh, Xp
• Applications: WB, ICC/IF, IHC, IP
• Clone: KR-10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

KDELR1 Antibody (KR-10) - BSA Free Summary

• Description: Novus Biologicals Mouse KDELR1 Antibody (KR-10) - BSA Free (NBP2-12873) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-KDELR1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
• Localization: Endoplasmic Reticulum
• Specificity: Detects approx 25kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KDELR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: 1 ug/ml was sufficient for detection of KDEL receptor in 20 ug monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Reactivity Notes

Use in Mammal reported in scientific literature (PMID:31653700).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for KDELR1 Antibody (KR-10) - BSA Free

• ER lumen protein retaining receptor 1
• ERD2
• ERD2.1KDEL receptor 1
• HDEL
• KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1
• KDEL endoplasmic reticulum protein retention receptor 1
• PM23
• Putative MAPK-activating protein PM23

Background

1ug/ml was sufficient for detection of KDEL receptor in 20ug monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG: AP as the secondary.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KDELR1 Antibody (NBP2-12873)(1)

Publication using NBP2-12873

• Bikard Y, Viviano J, Orr MN et al. The KDEL Receptor has a role in the Biogenesis and Trafficking of the Epithelial Sodium Channel (ENaC) J. Biol. Chem. 2019-10-25 [PMID: 31653700] (WB, Mammal)

Review for KDELR1 Antibody (NBP2-12873) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Boyan Zhang ICC Human 09/07/2021

Summary

• Application: Immunocytochemistry
• Sample Tested: RPE1 cells
• Species: Human
• Lot: mk386166

Bioinformatics

• Gene Symbol: KDELR1
• Uniprot:
  • Q99JH8(Mouse)