KDELR1 Antibody (KR-10) [DyLight 594]

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Ha, Ma, Pm, Rb, Sh, Xp
• Applications: WB, ICC/IF, IHC, IP
• Clone: KR-10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 594

KDELR1 Antibody (KR-10) [DyLight 594] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
• Localization: Endoplasmic Reticulum
• Specificity: Detects approx 25kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KDELR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-12873. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Use in Mammal reported in scientific literature (PMID:31653700).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for KDELR1 Antibody (KR-10) [DyLight 594]

• ER lumen protein retaining receptor 1
• ERD2
• ERD2.1KDEL receptor 1
• HDEL
• KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1
• KDEL endoplasmic reticulum protein retention receptor 1
• PM23
• Putative MAPK-activating protein PM23

Background

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KDELR1