KDELR1 Antibody (KR-10) [Alexa Fluor™ Plus 594]

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Ha, Ma, Pm, Rb, Sh, Xp
• Applications: WB, ICC/IF, IHC, IP
• Clone: KR-10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

KDELR1 Antibody (KR-10) [Alexa Fluor™ Plus 594] Summary

• Immunogen: A 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
• Localization: Endoplasmic Reticulum
• Specificity: Detects approx 25kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KDELR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Mammal reported in scientific literature (PMID:31653700).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for KDELR1 Antibody (KR-10) [Alexa Fluor™ Plus 594]

• ER lumen protein retaining receptor 1
• ERD2
• ERD2.1KDEL receptor 1
• HDEL
• KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1
• KDEL endoplasmic reticulum protein retention receptor 1
• PM23
• Putative MAPK-activating protein PM23

Background

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KDELR1