KCTD15 Antibody (AT4C3) - BSA Free

Western Blot: KCTD15 Antibody (AT4C3) [NBP1-49429] - The cell lysates (10ug) were resolved by SDS-PAGE,transferred to PVDF membrane and probed with anti-humanKCTD15 antibody (1:1000). Proteins were visualized using agoat anti-mouse secondary antibody conjugated to HRP andan ECL detection system.Lane 1 : 293T cell lysateLane 2 : KCTD15 Transfected 293T cell lysate

Immunocytochemistry/Immunofluorescence: KCTD15 Antibody (AT4C3) [NBP1-49429] - Human HeLa cells stained with monoclonal anti-human KCDT15 antibody (1:500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).

Western Blot: KCTD15 Antibody (AT4C3) [NBP1-49429] - PAGE, transferred to NC membrane and probed with anti-human KCTD15 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: AT4C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

KCTD15 Antibody (AT4C3) - BSA Free Summary

• Description: Novus Biologicals Mouse KCTD15 Antibody (AT4C3) - BSA Free (NBP1-49429) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human KCTD15 (1-234aa) purified from E. coli.
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KCTD15
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Western Blot 1:250-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for KCTD15 Antibody (AT4C3) - BSA Free

• BTB/POZ domain-containing protein KCTD15
• MGC25497
• MGC2628
• potassium channel tetramerisation domain containing 15

Background

KCTD15 (Potassium channel tetramerisation domain containing 15, also known as BTB/POZ domain-containing protein KCTD15) is protein that in humans is encoded by the KCTD15 gene. KCTD15 is expressed at high level in brain and hypothalamus. The potassium channel KCTD15 was identified as a genetic loci associated with higher than normal body mass index (BMI) in humans along with genes such as GNPDA2, MTCH2, FTO, and TMEM18. Single nucleotide polymorphisms (SNPs) in non-diabetic and diabetic patients showed that FTO was most strongly associated with obesity while MTCH2 and GNPDA2 were still significantly associated with higher than normal BMI levels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KCTD15
• Entrez:
  • 79047(Human)
• Uniprot:
  • NP_076981(Human)