Potassium channel, inwardly rectifying subfamily J member 10
potassium inwardly-rectifying channel, subfamily J, member 10
This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for KCNJ10 Antibody (NBP1-70371). (Showing 1 - 1 of 1 FAQ).
Which specific peptide sequence are detected by this antibody? It is only stated in the data sheet that it reacts against a part of the C-terminal part of KIR4.1. Also, do you know if this antibody, or any of your other antibodies, could be utilized as an intracellular staining of cells for flow cytometry? Furthermore, I have seen in the data sheet for the synthetic peptide that it is not suitable for use with live cells, but is this an absolute? If this is the case, would it possible to buy a similar peptide which could be used with live cells, and if so at which prize/amount? Lastly, are the peptide in a frozen liquid form (or solid powder) with out trace of azide? When it comes to the cell lysate I'm wondering if this could be utilized with live cells or if the RIPA buffer which it is dissolved in makes this very toxic? If the last is the case, is it possible to provide the lysate in another buffer which are appropriate for live cells? Lastly, I wondered if the transfected HEK293T cells are also sold for culture?
I can disclose that the immunogen sequence of this antibody corresponds to a region within amino acids 330-379 of human KCNJ10. This antibody has not yet been tested in flow cytometry (you can see this as flow is not listed as a tested application in the product information), so we cannot guarantee that it would be suitable for this application. However, if you would be interested in testing this novel application, please take a look at our Innovator's Reward program. I have searched for an alternative product for you, but unfortunately none of our KCNJ10 antibodies currently have flow cytometry as a validated application.
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