KCC2/SLC12A5 Antibody (S1-12) - BSA Free

Western Blot: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Western Blot analysis of Rat brain membrane lysate showing detection of KCC2/SLC12A5 protein using Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody, Clone S1-12 (NBP2-59337). Primary Antibody: Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody (NBP2-59337) at 1:1000.

Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody, Clone S1-12 (NBP2-59337). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-KCC2/SLC12A5 Monoclonal Antibody (NBP2-59337) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) KCC2/SLC12A5 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: KCC2/SLC12A5 Antibody (S1-12) [NBP2-59337] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-KCC2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane. Magnification: 60X.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: S1-12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

KCC2/SLC12A5 Antibody (S1-12) - BSA Free Summary

• Description: Novus Biologicals Mouse KCC2/SLC12A5 Antibody (S1-12) - BSA Free (NBP2-59337) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-KCC2/SLC12A5 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein amino acids 932-1043 corresponding to rat KCC2
• Localization: Membrane
• Specificity: Detects 140kDa.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC12A5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:300
  • Immunoprecipitation
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for KCC2/SLC12A5 Antibody (S1-12) - BSA Free

• Electroneutral potassium-chloride cotransporter 2
• hKCC2
• KCC2
• KCC2K-Cl cotransporter 2
• KIAA1176erythroid K-Cl cotransporter 2
• Neuronal K-Cl cotransporter
• SLC12A5
• solute carrier family 12 (potassium/chloride transporter), member 5
• solute carrier family 12 member 5

Background

K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons. Alternative splicing results in two transcript variants encoding different isoforms. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KCC2/SLC12A5 Antibody (NBP2-59337)(2)

Publications using NBP2-59337

• Francis J Prael Iii, Kwangho Kim, Yu Du, Brittany D Spitznagel, Gary A Sulikowski, Eric Delpire, C David Weaver Discovery of Small Molecule KCC2 Potentiators Which Attenuate In Vitro Seizure-Like Activity in Cultured Neurons. Frontiers in cell and developmental biology 2022-04-04 [PMID: 35813195] (Immunohistochemistry, Rat)
• Luo Y, Zhang D et al. Dexamethasone protects against arsanilic acid induced rat vestibular dysfunction through the BDNF and JNK 1/2 signaling pathways. Mol Med Rep 2019-01-03 [PMID: 30628712] (IF/IHC, Rat)

Bioinformatics

• Gene Symbol: SLC12A5