katanin-p80 Antibody (OTI5C4) - Azide and BSA Free

Western Blot: katanin-p80 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71578] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY katanin-p80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-katanin-p8

Immunocytochemistry/Immunofluorescence: katanin-p80 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71578] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY katanin-p80.

Immunohistochemistry: katanin-p80 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71578] - Staining of paraffin-embedded Human colon tissue using anti-katanin-p80 mouse monoclonal antibody.

Flow Cytometry: katanin-p80 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71578] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-katanin-p80 antibody, and then analyzed by flow cytometry.

Immunohistochemistry: katanin-p80 Antibody (OTI5C4) - Azide and BSA Free [NBP2-71578] - Staining of paraffin-embedded Human Kidney tissue using anti-katanin-p80 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI5C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

katanin-p80 Antibody (OTI5C4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse katanin-p80 Antibody (OTI5C4) - Azide and BSA Free (NBP2-01383) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human KATNB1(NP_005877) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KATNB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 72.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for katanin-p80 Antibody (OTI5C4) - Azide and BSA Free

• KAT
• katanin (80 kDa)
• katanin p80 (WD repeat containing) subunit B 1
• katanin p80 (WD40-containing) subunit B 1
• Katanin p80 subunit B1
• katanin p80 WD40-containing subunit B1
• p80 katanin

Background

Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help toorganize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemblemicrotubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family ofATPases. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KATNB1