KAP1 Antibody (1D11)

Western Blot: KAP1 Antibody (1D11) [H00010155-M02] - TRIM28 monoclonal antibody (M02), clone 1D11. Analysis of TRIM28 expression in NIH/3T3.

Immunohistochemistry-Paraffin: KAP1 Antibody (1D11) [H00010155-M02] - Analysis of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.

Western Blot: KAP1 Antibody (1D11) [H00010155-M02] - TRIM28 monoclonal antibody (M02), clone 1D11 Western Blot analysis of TRIM28 expression in Hela NE ( Cat # L013V3 ).

Western Blot: KAP1 Antibody (1D11) [H00010155-M02] - TRIM28 monoclonal antibody (M02), clone 1D11. Analysis of TRIM28 expression in PC-12.

Western Blot: KAP1 Antibody (1D11) [H00010155-M02] - TRIM28 monoclonal antibody (M02), clone 1D11. Analysis of TRIM28 expression in MCF-7.

Western Blot: KAP1 Antibody (1D11) [H00010155-M02] - Analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody (M02), clone 1D11.Lane 1: TRIM28 transfected lysate (Predicted MW: 88.5 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

KAP1 Antibody (1D11) Summary

• Immunogen: TRIM28 (AAH04978, 379 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSGDDPYSSAEPHVSGVKRSRSGEGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIER
• Specificity: TRIM28 (1D11)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM28
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for KAP1 Antibody (1D11)

• E3 SUMO-protein ligase TRIM28
• EC 6.3.2.-
• FLJ29029
• KAP1
• KAP-1
• KAP1KRAB-associated protein 1
• KRIP-1
• Nuclear corepressor KAP-1
• RNF96
• RNF96KRAB-interacting protein 1
• TF1B
• TIF1B
• TIF1-beta
• TIF1BRING finger protein 96
• transcription intermediary factor 1-beta
• transcriptional intermediary factor 1-beta
• TRIM28
• tripartite motif containing 28
• tripartite motif-containing 28
• Tripartite motif-containing protein 28

Background

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM28
• Entrez:
  • 10155(Human)
• OMIM:
  • 601742(Human)
• Uniprot:
  • AAH04978(Human)