Kallikrein 7 Antibody - BSA Free

Western Blot: Kallikrein 7 Antibody [NBP1-31428] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Kallikrein 7 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Kallikrein 7 Antibody [NBP1-31428] - Mouse skin. Kallikrein 7 stained by Kallikrein 7 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: Kallikrein 7 Antibody [NBP1-31428] - Cal27 xenograft, using KLK7 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Kallikrein 7 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Kallikrein 7 Antibody - BSA Free (NBP1-31428) is a polyclonal antibody validated for use in IHC and WB. Anti-Kallikrein 7 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Kallikrein 7. The exact sequence is proprietary.
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KLK7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Dog (82%), Pig (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Kallikrein 7 Antibody - BSA Free

• EC 3.4.21
• EC 3.4.21.117
• hK7
• hSCCE
• kallikrein 7 (chymotryptic, stratum corneum)
• Kallikrein 7
• kallikrein-related peptidase 7
• KLK7
• protease, serine, 6
• PRSS6
• SCCEkallikrein-7
• Serine protease 6
• signal protein
• Stratum corneum chymotryptic enzyme

Background

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded enzyme is thought to be involved in the proteolysis of intercellular cohesive structures preceding desquamation, which is the shedding of the outermost layer of the epidermis. Alternative splicing of this gene results in two transcript variants encoding the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kallikrein 7 Antibody (NBP1-31428)(3)

Publications using NBP1-31428

• Somogyi O, Dajnoki Z, Szab� L et al. New Data on the Features of Skin Barrier in Hidradenitis Suppurativa Biomedicines 2023-01-04 [PMID: 36672635] (Immunohistochemistry, Human)
• McGovern JA, Meinert C, de Veer SJ et al. Attenuated kallikrein-related peptidase activity disrupts desquamation and leads to stratum corneum thickening in human skin equivalent models. Br J Dermatol 2016-07-21 [PMID: 27442805]
• Sheets AR, Massey CJ, Cronk SM et al. Matrix- and plasma-derived peptides promote tissue-specific injury responses and wound healing in diabetic swine J Transl Med 2016-07-02 [PMID: 27369317] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: KLK7