| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P |
| Clone | SPM352 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 488 |
| Immunogen | PSA from human sperm plasma |
| Localization | Cytoplasmic |
| Specificity | Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KLK3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes) |
| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A purified |
![Kallikrein 3/PSA Antibody (SPM352) [DyLight 488]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Kallikrein 3/PSA Antibody (SPM352) [DyLight 488]")
Secondary Antibodies |
Isotype Controls |
Diseases for Kallikrein 3/PSA Antibody (NBP2-34768G)Discover more about diseases related to Kallikrein 3/PSA Antibody (NBP2-34768G).
| Pathways for Kallikrein 3/PSA Antibody (NBP2-34768G)View related products by pathway.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi](http://images.novusbio.com/fullsize2/Androgen R-NR3C4 [p Ser213- p Ser210] Antibody (156C135.2)-Western Blot-NB100-56603-img0003.jpg)
![Western Blot: AKR1B1 Antibody [NBP1-89146] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize2/AKR1B1-Antibody-Western-Blot-NBP1-89146-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody [NBP1-89146] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize2/AKR1B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89146-img0006.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.](http://images.novusbio.com/fullsize2/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0007.jpg)
![Western Blot: Ferredoxin Reductase Antibody [NBP2-38530] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize2/Ferredoxin-Reductase-Antibody-Western-Blot-NBP2-38530-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human cell line U-2 OS shows positivity in mitochondria.](http://images.novusbio.com/fullsize2/Ferredoxin-Reductase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38530-img0001.jpg)
![Immunohistochemistry: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human ovary shows strong cytoplasmic positivity in follicle cells.](http://images.novusbio.com/fullsize2/Ferredoxin-Reductase-Antibody-Immunohistochemistry-NBP2-38530-img0002.jpg)
![Western Blot: Adenine Nucleotide Translocator 2 Antibody [NBP2-56762] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize2/Adenine Nucleotide Translocator 2 Antibody-Western Blot-NBP2-56762-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Adenine Nucleotide Translocator 2 Antibody [NBP2-56762] - Staining of human cell line PC-3 shows localization to mitochondria.](http://images.novusbio.com/fullsize2/Adenine-Nucleotide-Translocator-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56762-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Analysis of phosphorylated ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.](http://images.novusbio.com/fullsize2/ASAP1 [p Tyr782] Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77933-img0005.jpg)
![Immunohistochemistry: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Used at 20 g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize2/ASAP1-[p-Tyr782]-Antibody-Immunohistochemistry-NBP1-77933-img0004.jpg)
![Western Blot: Reg3a Antibody [NBP2-24763] - Human intestine lysate probed with REG3A antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize2/Reg3a-Antibody-Western-Blot-NBP2-24763-img0004.jpg)
![Immunohistochemistry-Paraffin: Reg3a Antibody [NBP2-24763] - Formalin-fixed, paraffin-embedded human intestine stained with REG3A antibody (2.5 ug/ml), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize2/Reg3a-Antibody-Immunohistochemistry-Paraffin-NBP2-24763-img0003.jpg)
![Western Blot: PAG3 Antibody [NBP2-24614] - Analysis of ASAP2 in human brain lysate using 1) preimmune sera at 1:5000 and 2) protein A purified this antibody. 1.0 ug/ml.](http://images.novusbio.com/fullsize2/PAG3-Antibody-Western-Blot-NBP2-24614-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PAPOLA Antibody [NBP1-87345] - Staining of human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/PAPOLA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87345-img0010.jpg)
![Immunohistochemistry-Paraffin: PAPOLA Antibody [NBP1-87345] - Staining of human prostate shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize2/PAPOLA-Antibody-Immunohistochemistry-Paraffin-NBP1-87345-img0009.jpg)
![Western Blot: MRPS30 Antibody [NBP1-87343] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize2/MRPS30-Antibody-Western-Blot-NBP1-87343-img0006.jpg)
![Immunohistochemistry-Paraffin: MRPS30 Antibody [NBP1-87343] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize2/MRPS30-Antibody-Immunohistochemistry-Paraffin-NBP1-87343-img0007.jpg)
![Western Blot: MRPS30 Antibody [NBP1-87343] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize2/MRPS30-Antibody-Western-Blot-NBP1-87343-img0005.jpg)
![Western Blot: PDAP1 Antibody [NBP2-38621] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize2/PDAP1 Antibody-Western Blot-NBP2-38621-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: PDAP1 Antibody [NBP2-38621] - Staining of human cell line U-251 MG shows positivity in plasma membrane and cytoplasm.](http://images.novusbio.com/fullsize2/PDAP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38621-img0001.jpg)
![Immunohistochemistry-Paraffin: PDAP1 Antibody [NBP2-38621] - Staining of human tonsil shows cytoplasmic positivity mainly in germinal center cells.](http://images.novusbio.com/fullsize2/PDAP1-Antibody-Immunohistochemistry-Paraffin-NBP2-38621-img0004.jpg)
![Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - PSMA expression in human prostate cancer cell lines. Image from verified customer review.](http://images.novusbio.com/fullsize2/PSMA-FOLH1-NAALADase-I-Antibody-(GCP-04)-Western-Blot-NBP1-45057-img0010.jpg)
![Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - GCPII in porcine kidney by GCP-04 monoclonal antibody. Highly positive proximal glomeruli.](http://images.novusbio.com/fullsize2/PSMA-FOLH1-NAALADase-I-Antibody-(GCP-04)-Immunohistochemistry-Paraffin-NBP1-45057-img0008.jpg)
![Western Blot: PSMA/FOLH1/NAALADase I Antibody (GCP-04) [NBP1-45057] - Fragment of human GCPII (aminoacids 44-750) produced in S2 cells on Western blot by GCP-04 monoclonal antibody. Lanes 1, 2, 3 represent 800, 400 and 200 pg of the protein.](http://images.novusbio.com/fullsize2/PSMA-FOLH1-NAALADase-I-Antibody-(GCP-04)-Western-Blot-NBP1-45057-img0009.jpg)
![Western Blot: ACAT1 Antibody [NBP1-89285] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize2/ACAT1-Antibody-Western-Blot-NBP1-89285-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ACAT1 Antibody [NBP1-89285] - Staining of human cell line A-431 shows positivity in mitochondria.](http://images.novusbio.com/fullsize2/ACAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89285-img0003.jpg)
![Immunohistochemistry-Paraffin: ACAT1 Antibody [NBP1-89285] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize2/ACAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-89285-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: POMT1 Antibody [NBP2-57200] - Staining of human cell line MCF7 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize2/POMT1 Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57200-img0001.jpg)
![Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate. Lane2:HEK293T cell lysate. Lane3:Rat testis tissue lysate.](http://images.novusbio.com/fullsize2/APC-Antibody-Western-Blot-NB100-91662-img0004.jpg)
![Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.](http://images.novusbio.com/fullsize2/APC-Antibody-Immunohistochemistry-Paraffin-NB100-91662-img0005.jpg)
![Western Blot: APC Antibody [NB100-91662] - Extracts from COLO205 cells.](http://images.novusbio.com/fullsize2/APC Antibody-Western Blot-NB100-91662-img0001.jpg)
![ELISA: Kallikrein 3/PSA ELISA Kit [HRP] [KA0208] - Standard curve](http://images.novusbio.com/images2/Kallikrein-3-PSA-ELISA-Kit-[HRP]-ELISA-KA0208-img0001.jpg "ELISA: Kallikrein 3/PSA ELISA Kit [HRP] [KA0208] - Standard curve")
![Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi](http://images.novusbio.com/images2/Androgen R-NR3C4 [p Ser213- p Ser210] Antibody (156C135.2)-Western Blot-NB100-56603-img0003.jpg "Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi")