Kallikrein 3/PSA Antibody (CL9422) - Azide and BSA Free

Analysis in human prostate and placenta tissues using NBP3-44048 antibody. Corresponding Kallikrein 3/PSA RNA-seq data are presented for the same tissues.

Staining of human placenta shows no positivity in trophoblastic cells as expected.

Staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Staining of human prostate cancer shows strong cytoplasmic positivity in tumor cells.

Analysis in human prostate tissue.

Staining of human tonsil shows no positivity in non-germinal and germinal center cells as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL9422
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Kallikrein 3/PSA Antibody (CL9422) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of P07288, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KLK3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Kallikrein 3/PSA Antibody (CL9422) - Azide and BSA Free

• APS seminin
• EC 3.4.21
• EC 3.4.21.77
• Gamma-seminoprotein
• hK3
• Kallikrein 3
• kallikrein 3, (prostate specific antigen)
• Kallikrein-3
• kallikrein-related peptidase 3
• KLK2A1
• KLK3
• P-30 antigen
• prostate specific antigen
• prostate-specific antigen
• PSA semenogelase
• PSA
• Semenogelase
• Seminin

Background

PSA is a chymotrypsin-like serine protease (kallikrein family) produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liverderived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLK3