Jumonji/JARID2 Antibody [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

Jumonji/JARID2 Antibody [DyLight 650] Summary

Immunogen
A synthetic peptide made to an N-terminal portion of the human JARID2 protein sequence (between residues 1-100). [UniProt# Q92833]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
JARID2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This JARID2 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry-paraffin embedded sections. Immunoprecipitation was reported in scientific literature. By Western blot a band is seen at ~140 kDa. Another band is seen >200 kDa and a faint band may be seen ~80 kDa. This antibody has also been reported to work in Chromatin Immunoprecipitation (ChIP), see PMID: 22396653. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nucleus of mouse brain. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 24074864)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Jumonji/JARID2 Antibody [DyLight 650]

  • JARID2
  • JMJ
  • JMJjumonji-like protein
  • jumonji (mouse) homolog
  • jumonji homolog
  • Jumonji
  • jumonji, AT rich interactive domain 2
  • Jumonji/ARID domain-containing protein 2
  • protein Jumonji

Background

Jumonji is a nuclear protein that participates in the negative regulation of cell growth. It is required for neural tube formation and is essential for normal heart development and function. As well, it is a part of a group of proteins characterized by a novel structural motif, the JmjC domain, which is implicated in chromatin regulation through histone demethylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB100-2214B
DyLight 488 Labeled NB100-2214G
DyLight 550 Labeled NB100-2214R
HRP Labeled NB100-2214H

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Bioinformatics Tool for Jumonji/JARID2 Antibody (NB100-2214C)

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Bioinformatics

Gene Symbol JARID2
Entrez