JNK1/2/3 Antibody [Unconjugated] - Pan Specific

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands for JNK were detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

JNK was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

JNK was detected in perfusion fixed frozen sections of rat kidney using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for JNK at approximately 47 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Time course of JNK activation by combined UVR and thermal stresses in human fibroblasts and coral.Immunoblot revealing activated (pJNK) and total JNK (JNK) in (a) human fibroblasts or (b) S. pistillata nubbins exposed to UVR and temperature rise for the indicated times. (a) Total JNK and actin served as loading controls for human fibroblasts. (b) Total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28374828), licensed under a CC-BY license. Not internally tested by R&D Systems.

Time course of JNK activation by combined UVR and thermal stresses in human fibroblasts and coral.Immunoblot revealing activated (pJNK) and total JNK (JNK) in (a) human fibroblasts or (b) S. pistillata nubbins exposed to UVR and temperature rise for the indicated times. (a) Total JNK and actin served as loading controls for human fibroblasts. (b) Total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28374828), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of JNK activation in human fibroblasts and corals.Immunoblot revealing activated (pJNK) and total forms of JNK (JNK) present in (a) human fibroblasts and (b) S. pistillata nubbins incubated in the absence (control; ct) or presence of SP600125 (SP), anisomycin (Ani), or combination of both (Ani + SP). Molecular weight standards in kilodaltons (kDa) are indicated on the right side of the blots. (a) Total JNK and actin served as loading controls for human fibroblasts. (b) Total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28374828), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

JNK1/2/3 Antibody [Unconjugated] - Pan Specific Summary

• Immunogen: E. coli-derived recombinant human JNK1
  Met1-Gln384
  Accession # P45983
• Specificity: Detects human, mouse, and rat p46 and p54 JNK in Western blots. Detects recombinant human (rh) JNK1, rhJNK2, and rhJNK3.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK8IP3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JNK1/2/3 Antibody [Unconjugated] - Pan Specific

• c-Jun N-terminal kinase 1
• EC 2.7.11
• EC 2.7.11.24
• JNK
• JNK1 alpha protein kinase
• JNK1 beta protein kinase
• JNK1JNK1A2
• JNK-46
• JUN N-terminal kinase
• MAP kinase 8
• MAPK 8
• mitogen-activated protein kinase 8 isoform JNK1 alpha1
• mitogen-activated protein kinase 8 isoform JNK1 beta2
• mitogen-activated protein kinase 8
• PRKM8JNK
• protein kinase JNK1
• SAPK1JNK21B1/2
• Stress-activated protein kinase 1
• Stress-activated protein kinase JNK1

Background

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JNK1/2/3 Antibody (AF1387)(15)

Publications using AF1387

• Hiraide, S;Machida, T;Takihana, S;Ohshita, M;Iizuka, K; Pressure stress delays cyclooxygenase-2 expression induced by interleukin-1? in cultured human pulmonary artery smooth muscle cells Heliyon 2023-10-01 [PMID: 37876479] (Western Blot, Human)
• Qian Xia, Meng Mao, Gaofeng Zhan, Zhenzhao Luo, Yin Zhao, Xing Li SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury iScience 2023-06-16 [PMID: 37332598] (Western Blot, Mouse)
• Geou-Yarh Liou, Alicia K. Fleming Martinez, Heike R. Döppler, Ligia I. Bastea, Peter Storz Inflammatory and alternatively activated macrophages independently induce metaplasia but cooperatively drive pancreatic precancerous lesion growth iScience 2023-06-16 [PMID: 37250781] (Western Blot, Transgenic Mouse)
• Emilia Alors-Perez, Ricardo Blázquez-Encinas, Sonia Alcalá, Cristina Viyuela-García, Sergio Pedraza-Arevalo, Vicente Herrero-Aguayo et al. Dysregulated splicing factor SF3B1 unveils a dual therapeutic vulnerability to target pancreatic cancer cells and cancer stem cells with an anti-splicing drug Journal of Experimental & Clinical Cancer Research 2021-12-02 [PMID: 34857016]
• J Aguado, HK Chaggar, C Gómez-Incl, MR Shaker, HC Leeson, A Mackay-Sim, EJ Wolvetang Inhibition of the cGAS-STING pathway ameliorates the premature senescence hallmarks of Ataxia-Telangiectasia brain organoids Aging Cell, 2021-08-30;0(0):e13468. 2021-08-30 [PMID: 34459078] (Western Blot, Human)
• J Falero-Per, N Sheibani, CM Sorenson Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells PLoS ONE, 2020-05-04;15(5):e0232779. 2020-05-04 [PMID: 32365083] (Western Blot, Mouse)
• JM Jiménez-Va, V Herrero-Ag, AJ Montero-Hi, E Gómez-Góme, AC Fuentes-Fa, AJ León-Gonzá, P Sáez-Martí, E Alors-Pére, S Pedraza-Ar, T González-S, O Reyes, A Martínez-L, R Sánchez-Sá, S Ventura, EM Yubero-Ser, MJ Requena-Ta, JP Castaño, MD Gahete, RM Luque Dysregulation of the splicing machinery is directly associated to aggressiveness of prostate cancer EBioMedicine, 2020-01-03;51(0):102547. 2020-01-03 [PMID: 31902674] (Western Blot, Human)
• SA Barutcu, N Girnius, S Vernia, RJ Davis Role of the MAPK/cJun NH2-Terminal Kinase signaling pathway in starvation-induced autophagy Autophagy, 2018-08-17;0(0):. 2018-08-17 [PMID: 29950132] (Western Blot, Mouse)
• Nomeda Girnius, Yvonne JK Edwards, David S Garlick, Roger J Davis The cJUN NH2-terminal kinase (JNK) signaling pathway promotes genome stability and prevents tumor initiation eLife 2018-06-01 [PMID: 29856313]
• N Girnius, RJ Davis JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins Cell Rep, 2017-11-14;21(7):1910-1921. 2017-11-14 [PMID: 29141222] (Western Blot, Human)
• L Courtial, V Picco, R Grover, Y Cormerais, C Rottier, A Labbe, G Pagès, C Ferrier-Pa The c-Jun N-terminal kinase prevents oxidative stress induced by UV and thermal stresses in corals and human cells Sci Rep, 2017-04-04;7(0):45713. 2017-04-04 [PMID: 28374828] (Western Blot, Coral - Stylophora pistillata)
• Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C White-to-brown metabolic conversion of human adipocytes by JAK inhibition. Nat Cell Biol, 2014-12-08;17(1):57-67. 2014-12-08 [PMID: 25487280] (Western Blot, Human)
• Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema. Am. J. Physiol. Lung Cell Mol. Physiol., 2009-06-12;297(2):L388-400. 2009-06-12 [PMID: 19525388] (Western Blot, Human)
• Lopez R, Demick KP, Mansfield JM, Paulnock DM Type I IFNs play a role in early resistance, but subsequent susceptibility, to the African trypanosomes. J. Immunol., 2008-10-01;181(7):4908-17. 2008-10-01 [PMID: 18802094] (Western Blot, Mouse)
• Kato T, Noma H, Kitagawa M, Takahashi T, Oshitani N, Kitagawa S Distinct role of c-Jun N-terminal kinase isoforms in human neutrophil apoptosis regulated by tumor necrosis factor-alpha and granulocyte-macrophage colony-stimulating factor. J. Interferon Cytokine Res., 2008-04-01;28(4):235-43. 2008-04-01 [PMID: 18439101] (Western Blot, Human)

Bioinformatics

• Gene Symbol: MAPK8IP3
• Uniprot:
  • P45983()