JNK1/2/3 Antibodies: Novus Biologicals

AF1205

JNK1/2/3 [p Thr183, p Tyr185] Antibody [Unconjugated]

(1 Review)

50 μg

AF1205

HAF008). Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of HEK293 human embryonic kidney cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 J/m<SUP>2</SUP>UV-C for 30 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1205), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 J/m²UV-C for 30 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1205), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6." class="big_thumb" />

CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." class="big_lightbox">

<b>Biological Strategies Validation. </b>JNK phosphorylated at T183/Y185 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1205) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." class="big_thumb" />

CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

JNK phosphorylated at T183/Y185 was detected in immersion fixed paraffin-embedded sections of human prostate array using Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1205) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100 J/m<SUP>2</SUP>ultraviolet light (UV) for 30 minutes, loaded at 0.2 mg/mL. Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 56 and 46 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1205). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.</PP>*Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

<b>Biological Strategies Validation. </b>Effect of p38MAPK (p38) inhibition on intracellular signaling following IR. Rats were pretreated with the carrier DMSO or BIRB796 (B-796) (5 mg/kg BW) for 1 hour and subjected to 1 hour of renal ischemia followed by different time points of reperfusion (15 min, 2 days, 7 days). Kidneys were harvested at given time points of reperfusion and total tissue lysates were used to determine activation pattern of MAPKs (p38MAPK, JNK, ERK) and the downstream target of p38MAPK (MK2) by phosphorylation specific antibodies. A representative immunoblot (A) and summary graphs (B, C) are shown. Results are given as mean ± SEM (n = 3). ** p < 0.01, ***p < 0.001 vs. sham-operated group; §p < 0.01, #p < 0.001 vs. IR-15 min group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24423080), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Inhibition of JNK kinases prevents p66ShcS36 phosphorylation under oxidative stress.MEFs were treated with H2O2 (a) (1 mM, 30 min) or exposed to HR (hypoxia 90 min, reoxygenation 15 min) (b). Cell lysates were probed with the antibodies indicated to assess p66Shc phosphorylation at S36. Representative Western blots (individual experiment performed under the same experimental conditions and run on the same gel) and summary graphs from at least three independent experiments are shown. Signal intensity of control samples was arbitrarily set at 1. Mitochondrial ROS production was measured in p66Shc+/+ and p66Shc−/− MEFs after prooxidant treatment as described in Material and Methods (n ≥ 4) (c) and phospho gamma H2AX was detected by immunoblotting (d, lower panel) and summary graph is shown in above panel. SP: SP600125, JNK inhibitor, and BR: BR796, p38 inhibitor were applied 1 h prior to stress application. Original blot shown in (b) have been cropped and the full length blots are shown in the Supplementary Figure S1b Statistical analysis was performed using t-test or ANOVA (*p < 0.05 **p < 0.01, ***p < 0.001). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26868434), licensed under a CC-BY license. Not internally tested by R&D Systems.

The impact of high glucose conditions on various down-stream signaling pathways.The status of various signaling pathways in retinal AC under various glucose conditions was determined as described in Methods. Levels of active Src (A), Akt (B), ERK (C), p38 (D), JNK1 (E), Fyn (F) and p65 (G) in retinal AC under different glucose conditions were determined by Western blot analysis from equal amounts of cell lysates. The total level for each protein is shown in the lower part of each panel. The quantitative assessment of the data for each blot is shown below the blot. Data are presented as mean ± SEM. n = 3; *p<0.05 (NG vs. HG and NG vs. NG+L-Glu). Levels of active Fyn (F) in retinal AC under different glucose conditions was determined by Western blot analysis of immunoprecipitates from equal amounts of retinal AC lysates. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0103148), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inhibition of JNK kinases prevents p66ShcS36 phosphorylation under oxidative stress.MEFs were treated with H2O2 (a) (1 mM, 30 min) or exposed to HR (hypoxia 90 min, reoxygenation 15 min) (b). Cell lysates were probed with the antibodies indicated to assess p66Shc phosphorylation at S36. Representative Western blots (individual experiment performed under the same experimental conditions and run on the same gel) and summary graphs from at least three independent experiments are shown. Signal intensity of control samples was arbitrarily set at 1. Mitochondrial ROS production was measured in p66Shc+/+ and p66Shc−/− MEFs after prooxidant treatment as described in Material and Methods (n ≥ 4) (c) and phospho gamma H2AX was detected by immunoblotting (d, lower panel) and summary graph is shown in above panel. SP: SP600125, JNK inhibitor, and BR: BR796, p38 inhibitor were applied 1 h prior to stress application. Original blot shown in (b) have been cropped and the full length blots are shown in the Supplementary Figure S1b Statistical analysis was performed using t-test or ANOVA (*p < 0.05 **p < 0.01, ***p < 0.001). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26868434), licensed under a CC-BY license. Not internally tested by R&D Systems.

Activation of ERK1/2 signaling was necessary for venlafaxine-induced apoptosis. (A) Representative western-blot bands and quantification of the MAPK signal molecules abundances in MV3 cells treated with venlafaxine (10 μM) for 0–8 h (B) MV3 cells were treated with vehicle, JNK1/2 inhibitor SP600125 and MERK/ERK inhibitor PD98059 for 72 h. Cell viability were measured by CCK-8 kits at 72 h (C) MV3 cells were transfected with siRNA control, JNK1/2 siRNA, and ERK siRNA for 72 h. Cell viability were measured by CCK-8 kits at 72 h (D,E) Representative western-blot bands and quantification of Nur77 abundances in MV3 cells treated with venlafaxine (10 μM) for 8 h, or transfected with siRNA control, JNK1/2 siRNA, and ERK siRNA for 20 h (F) MV3 cells were immunostained with Bcl-2 and Nur77 antibodies and visualized by confocal microscopy. N = 3, *, p <.05, **, <.01, ***, p <.001 vs. control. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36686679), licensed under a CC-BY license. Not internally tested by R&D Systems.

Evaluation of the phosphorylation of MAPK family members and Raf-1 in Bag-1-overexpressing NP cells. Western blot analysis (A) and quantification (B,C) of the phosphorylated forms of p38, ERK, JNK, and Raf-1 in NP cells treated with or without H2O2 for 30 min. Data are the means ± SD n = 3, * p < 0.05. (D) Comparison of the expression of pERK in NP cells (NP) and Bag-1-overexpressing NP cells (Bag) without H2O2 treatment. Data are the means ± SD n = 3, * p < 0.05. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/2227-9059/11/3/863), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1205-SP

compare

(select up to 3 products from this page to compare)

AF1387

JNK1/2/3 Antibody [Unconjugated] - Pan Specific

(16 Publications)

100 μg

AF1387

HAF008). Specific bands for JNK were detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). Specific bands for JNK were detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands for JNK were detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." class="big_lightbox">

JNK was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." class="big_thumb" />

CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

JNK was detected in perfusion fixed frozen sections of rat kidney using Rabbit Anti-Human/Mouse/Rat JNK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for JNK at approximately 47 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat JNK Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1387) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Time course of JNK activation by combined UVR and thermal stresses in human fibroblasts and coral.Immunoblot revealing activated (pJNK) and total JNK (JNK) in (a) human fibroblasts or (b) S. pistillata nubbins exposed to UVR and temperature rise for the indicated times. (a) Total JNK and actin served as loading controls for human fibroblasts. (b) Total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28374828), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of JNK activation in human fibroblasts and corals.Immunoblot revealing activated (pJNK) and total forms of JNK (JNK) present in (a) human fibroblasts and (b) S. pistillata nubbins incubated in the absence (control; ct) or presence of SP600125 (SP), anisomycin (Ani), or combination of both (Ani + SP). Molecular weight standards in kilodaltons (kDa) are indicated on the right side of the blots. (a) Total JNK and actin served as loading controls for human fibroblasts. (b) Total JNK and amido black staining (a,b) served as loading controls for S. pistillata. Data are representative of at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28374828), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1387U-100UG
Alexa Fluor 405	AF1387V-100UG
Alexa Fluor 488	AF1387G-100UG
Alexa Fluor 532	AF1387X-100UG
Alexa Fluor 594	AF1387T-100UG
Alexa Fluor 647	AF1387R-100UG
Alexa Fluor 700	AF1387N-100UG
Alexa Fluor 750	AF1387S-100UG
Alexa Fluor Plus 405	AF1387AFP405-100UG
Alexa Fluor Plus 488	AF1387AFP488-100UG
Alexa Fluor Plus 555	AF1387AFP555-100UG
Alexa Fluor Plus 594	AF1387AFP594-100UG
Alexa Fluor Plus 647	AF1387AFP647-100UG
Alexa Fluor Plus 680	AF1387AFP680-100UG
Allophycocyanin	AF1387A
Biotin	AF1387B
CoraFluor 1	AF1387CL1
DyLight 350	AF1387D
DyLight 405	AF1387E
DyLight 488	AF1387K
DyLight 550	AF1387L
DyLight 594	AF1387M
DyLight 650	AF1387W
DyLight 680	AF1387Y
DyLight 755	AF1387Z
FITC	AF1387F
HRP	AF1387H
Janelia Fluor 525	AF1387JF525
Janelia Fluor 549	AF1387I
Janelia Fluor 646	AF1387J
Janelia Fluor 669	AF1387JF669
PE	AF1387P
PerCP	AF1387C
mFluor Violet 450 SE	AF1387MFV450
mFluor Violet 500 SE	AF1387MFV500
mFluor Violet 610 SE	AF1387MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1387-SP

compare

(select up to 3 products from this page to compare)

MAB1205

JNK1/2/3 [p Thr183, p Tyr185] Antibody (1006A) [Unconjugated]

(1 Review)

(10 Publications)

100 μg

MAB1205

HAF008). Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and 293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm<sup>2</sup>ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

HAF008). Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and 293T human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm²ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/ml of Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-JNK (T183/Y185) at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL004) and counterstained with DAPI. Filamentous actin was stained with fluorescein-conjugated phalloidin (green). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

<b>Biological Strategies Validation. </b>JNK phosphorylated at T183/Y185 was detected in immersion fixed HEK293 human embryonic kidney cell line untreated (lower panel) or treated with UV radiation (upper panel) using Rabbit Anti-Human/Mouse/Rat Phospho-JNK (T183/Y185) Monoclonal Antibody (Catalog # MAB1205) at 25 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # <a class=

NL004) and counterstained with DAPI. Filamentous actin was stained with fluorescein-conjugated phalloidin (green). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

<b>Biological Strategies Validation. </b>ACPA inhibits p-Akt, induces p-JNK and affects levels of specific metabolites in NSCLC lines.a Principal component analysis (PCA) score plot: Metabolomics profiling of control and ACPA-treated A549, H1299, H358, and H838 cells. b Changes in variable importance in projection (VIP) values for 19 metabolites in A549 cells. c, d, e Changes in VIP values for 20 metabolites in H1299, H358, and H838 cells. Significantly changed metabolites (*p < 0.05, indicated by arrows) were matched to apoptotic pathways. f, g, h, i Increase and decrease in several metabolites of ACPA-treated A549, H1299, H358, and H838 cells (*p < 0.05). j Simple Western showing total Akt, p-Akt (S473), total JNK46 and JNK54 and p-JNK46 and p-JNK54 (T183/Y185) in A549 cells at 24 hours after treatment with IC50 dose of ACPA. k Relative expression levels of Akt and p-Akt for control and ACPA-treated A549 cells after normalization by total vinculin protein. l Relative expression levels of JNK (46 and 54 kDa) and p-JNK for control and ACPA-treated A549 cells after normalization by total vinculin protein. *p < 0.05, Student’s t-test. All tests were done in quadruplicates. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33431819), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of LSW treatment on TNF alpha -mediated NF-kappa B and p38/JNK signaling in EA.hy926 cells. The cells were treated with LSW (50 μM) for 18 h before TNF alpha (10 ng/mL) stimulation for 15 min, followed by the detecting the protein expression of I kappa B alpha (A), p65 (B), p38 (C), and JNK (D) by Western blotting. Protein bands of I kappa B alpha were normalized to GAPDH; bands of the phosphorylated p65, p38, and JNK were normalized to their total forms. Data were normalized to the untreated group (Untr). *, p < 0.05; **, p < 0.01; ****, p < 0.0001, ns, not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36359987), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1205U-100UG
Alexa Fluor 405	FAB1205V-100UG
Alexa Fluor 488	FAB1205G-100UG
Alexa Fluor 532	FAB1205X-100UG
Alexa Fluor 594	FAB1205T-100UG
Alexa Fluor 647	FAB1205R-100UG
Alexa Fluor 700	FAB1205N-100UG
Alexa Fluor 750	FAB1205S-100UG
Alexa Fluor Plus 405	FAB1205AFP405-100UG
Alexa Fluor Plus 488	FAB1205AFP488-100UG
Alexa Fluor Plus 555	FAB1205AFP555-100UG
Alexa Fluor Plus 594	FAB1205AFP594-100UG
Alexa Fluor Plus 647	FAB1205AFP647-100UG
Alexa Fluor Plus 680	FAB1205AFP680-100UG
Allophycocyanin	FAB1205A
Biotin	FAB1205B
CoraFluor 1	FAB1205CL1
DyLight 350	FAB1205D
DyLight 405	FAB1205E
DyLight 488	FAB1205K
DyLight 550	FAB1205L
DyLight 594	FAB1205M
DyLight 650	FAB1205W
DyLight 680	FAB1205Y
DyLight 755	FAB1205Z
FITC	FAB1205F
HRP	FAB1205H
Janelia Fluor 525	FAB1205JF525
Janelia Fluor 549	FAB1205I
Janelia Fluor 646	FAB1205J
Janelia Fluor 669	FAB1205JF669
PE	FAB1205P
PerCP	FAB1205C
mFluor Violet 450 SE	FAB1205MFV450
mFluor Violet 500 SE	FAB1205MFV500
mFluor Violet 610 SE	FAB1205MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1205-SP

compare

(select up to 3 products from this page to compare)

MAB1387

JNK1/2/3 Antibody (252355) [Unconjugated] - Pan Specific

(5 Publications)

100 μg

MAB1387

HAF007). Specific bands for JNK were detected at approximately 46 kDa (p46 JNK) and 54 kDa (p54 JNK) (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK Pan Specific Monoclonal Antibody (Catalog # MAB1387) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). Specific bands for JNK were detected at approximately 46 kDa (p46 JNK) and 54 kDa (p54 JNK) (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK Pan Specific Monoclonal Antibody (Catalog # MAB1387) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands for JNK were detected at approximately 46 kDa (p46 JNK) and 54 kDa (p54 JNK) (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK Pan Specific Monoclonal Antibody (Catalog # MAB1387) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1387U-100UG
Alexa Fluor 405	FAB1387V-100UG
Alexa Fluor 488	FAB1387G-100UG
Alexa Fluor 532	FAB1387X-100UG
Alexa Fluor 594	FAB1387T-100UG
Alexa Fluor 647	FAB1387R-100UG
Alexa Fluor 700	FAB1387N-100UG
Alexa Fluor 750	FAB1387S-100UG
Alexa Fluor Plus 405	FAB1387AFP405-100UG
Alexa Fluor Plus 488	FAB1387AFP488-100UG
Alexa Fluor Plus 555	FAB1387AFP555-100UG
Alexa Fluor Plus 594	FAB1387AFP594-100UG
Alexa Fluor Plus 647	FAB1387AFP647-100UG
Alexa Fluor Plus 680	FAB1387AFP680-100UG
Allophycocyanin	FAB1387A
Biotin	FAB1387B
CoraFluor 1	FAB1387CL1
DyLight 350	FAB1387D
DyLight 405	FAB1387E
DyLight 488	FAB1387K
DyLight 550	FAB1387L
DyLight 594	FAB1387M
DyLight 650	FAB1387W
DyLight 680	FAB1387Y
DyLight 755	FAB1387Z
FITC	FAB1387F
HRP	FAB1387H
Janelia Fluor 525	FAB1387JF525
Janelia Fluor 549	FAB1387I
Janelia Fluor 646	FAB1387J
Janelia Fluor 669	FAB1387JF669
PE	FAB1387P
PerCP	FAB1387C
mFluor Violet 450 SE	FAB1387MFV450
mFluor Violet 500 SE	FAB1387MFV500
mFluor Violet 610 SE	FAB1387MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1387-SP

compare

(select up to 3 products from this page to compare)

NBP3-16148

JNK1/2/3 Antibody - Azide and BSA Free

100 ul

NBP3-16148

Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded mouse spleen using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded human colon using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunohistochemistry analysis of paraffin-embedded mouse brain using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence: JNK1/2/3 Antibody - Azide and BSA Free [NBP3-16148] - Immunofluorescence analysis of U2OS cells using JNK1/2/3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-16148-20ul

compare

(select up to 3 products from this page to compare)

NBP3-21529

JNK1/2/3 [p Thr183] Antibody (SR1788) - BSA Free

100 ul

NBP3-21529

<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: JNK1/2/3 [p Thr 183] Antibody (SR1788) [NBP3-21529] - Immunofluorescent analysis of NIH/3T3 cells treated with Anisomycin, using NBP3-21529.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21529-50ul

compare

(select up to 3 products from this page to compare)

NBP3-21671

JNK1/2/3 Antibody (SR1787) - BSA Free

100 ul

NBP3-21671

Reactivity:

Hu, Mu, Rt, Bv, Ca, Pm, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Pm, Ze

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-21671-50ul

compare

(select up to 3 products from this page to compare)

NBP3-16250

JNK1/2/3 Antibody (0J5D8)

100 ul

NBP3-16250

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

FAB1205AFP594

JNK1/2/3 Antibody (1006A) [Alexa Fluor™ Plus 594]

100 μg

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Alexa Fluor Plus 594

compare

(select up to 3 products from this page to compare)

FAB1387AFP680

JNK1/2/3 Antibody (252355) [Alexa Fluor™ Plus 680]

100 μg

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 680

compare

(select up to 3 products from this page to compare)

FAB1205AFP405

JNK1/2/3 Antibody (1006A) [Alexa Fluor™ Plus 405]

100 μg

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Alexa Fluor Plus 405

compare

(select up to 3 products from this page to compare)

FAB1387AFP647

JNK1/2/3 Antibody (252355) [Alexa Fluor™ Plus 647]

100 μg

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 647

compare

(select up to 3 products from this page to compare)

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

JNK1/2/3 Antibodies

JNK1/2/3 [p Thr183, p Tyr185] Antibody [Unconjugated]

JNK1/2/3 Antibody [Unconjugated] - Pan Specific

JNK1/2/3 [p Thr183, p Tyr185] Antibody (1006A) [Unconjugated]

JNK1/2/3 Antibody (252355) [Unconjugated] - Pan Specific

JNK1/2/3 Antibody - Azide and BSA Free

JNK1/2/3 [p Thr183] Antibody (SR1788) - BSA Free

JNK1/2/3 Antibody (SR1787) - BSA Free

JNK1/2/3 Antibody (0J5D8)

JNK1/2/3 Antibody (1006A) [Alexa Fluor™ Plus 594]

JNK1/2/3 Antibody (252355) [Alexa Fluor™ Plus 680]

JNK1/2/3 Antibody (1006A) [Alexa Fluor™ Plus 405]

JNK1/2/3 Antibody (252355) [Alexa Fluor™ Plus 647]