JMJD5 Antibody

Images

 
Western Blot: JMJD5 Antibody [NBP1-77074] - Human liver tissue lysate with JMJD5 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: JMJD5 Antibody [NBP1-77074] - Immunofluorescence of JMJD5 in Rat Liver cells with JMJD5 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: JMJD5 Antibody [NBP1-77074] - Rat liver tissue with JMJD5 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

JMJD5 Antibody Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human JMJD5. The immunogen is located within amino acids 350 - 400 of JMJD5.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
47.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
JMJD5 Peptide (NBP1-77074PEP)
Reviewed Applications
Read 1 Review rated 3
using
NBP1-77074 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for JMJD5 Antibody

  • EC 1.14.11.27
  • FLJ13798
  • JmjC domain-containing protein 5
  • jumonji domain containing 5
  • Jumonji domain-containing protein 5
  • KDM8
  • lysine-specific demethylase 8

Background

The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. While several JMJD proteins have been identified as being involved in chromatin regulation, histone demethylation and development, the function of JMJD5 has not been identified. JMJD5 is expressed in multiple tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for JMJD5 Antibody (NBP1-77074) (0)

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Review for JMJD5 Antibody (NBP1-77074) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-77074:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 09/07/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Concentration1:2000

Details

Detection NotesRegular Protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for JMJD5 Antibody (NBP1-77074) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Discover related pathways, diseases and genes to JMJD5 Antibody (NBP1-77074). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

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Anonymous
09/07/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol KDM8
Entrez
Uniprot