JMJD1B Antibody

Immunoprecipitation: JMJD1B Antibody [NBP2-17007] - IP samples: NT2D1 whole cell extract A. 50 ug NT2D1 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of KDM3B protein by 4 ug KDM3B antibody [C2C3], C-term 5 % SDS-PAGE

Western Blot: JMJD1B Antibody [NBP2-17007] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with KDM3B antibody [C2C3], C-term (GTX116198) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: JMJD1B Antibody [NBP2-17007] -NT2D1 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM3B antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.

Western Blot: JMJD1B Antibody [NBP2-17007] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with JMJD1B antibody [C2C3], C-term (NBP2-17007) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: JMJD1B Antibody [NBP2-17007] - NT2D1 whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with JMJD1B antibody [C2C3], C-term (NBP2-17007) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

JMJD1B Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human JMJD1B. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM3B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 192 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for JMJD1B Antibody

• C5orf7
• EC 1.14.11
• EC 1.14.11.-
• EC 1.14.11.65
• JHDM2B
• JmjC domain-containing histone demethylation protein 2B
• JMJD1B
• jumonji domain containing 1B
• Jumonji domain-containing protein 1B
• KDM3B
• KIAA1082
• KIAA1082chromosome 5 open reading frame 7
• lysine (K)-specific demethylase 3B
• lysine-specific demethylase 3B
• NET22,5qNCA
• Nuclear protein 5qNCA

Background

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: KDM3B
• Uniprot:
  • Q7LBC6(Human)