JAM-B/VE-JAM Antibody (1G4) - Azide and BSA Free

Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - JAM2 monoclonal antibody (M01), clone 1G4. Analysis of JAM2 expression in human ovarian cancer.

Immunohistochemistry-Paraffin: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - Analysis of monoclonal antibody to JAM2 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 1 ug/ml.

Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - JAM2 expression in LNCaP ( Cat # L004V1 ).

Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - JAM2 monoclonal antibody (M01), clone 1G4. Analysis of JAM2 expression in HL-60.

Western Blot: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - Analysis of JAM2 expression in transfected 293T cell line by JAM2 monoclonal antibody (M01), clone 1G4.Lane 1: JAM2 transfected lysate (Predicted MW: 33.2 KDa).Lane 2: Non-transfected lysate.

ELISA: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - Detection limit for recombinant GST tagged JAM2 is approximately 3ng/ml as a capture antibody.

Sandwich ELISA: JAM-B/VE-JAM Antibody (1G4) [H00058494-M01] - Detection limit for recombinant GST tagged JAM2 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

JAM-B/VE-JAM Antibody (1G4) - Azide and BSA Free Summary

• Immunogen: JAM2 (AAH17779, 29 a.a. ~ 298 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FSAPKDQQVVTAVEYQEAILACKTPKKTVSSRLEWKKLGRSVSFVYYQQTLQGDFKNRAEMIDFNIRIKNVTRSDAGKYRCEVSAPSEQGQNLEEDTVTLEVLVAPAVPSCEVPSSALSGTVVELRCQDKEGNPAPEYTWFKDGIRLLENPRLGSQSTNSSYTMNTKTGTLQFNTVSKLDTGEYSCEARNSVGYRRCPGKRMQVDDLNISGIIAAVVVVALVISVCGLGVCYAQRKGYFSKETSFQKSNSSSKATTMSENDFKHTKSFII
• Specificity: JAM2 - junctional adhesion molecule 2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: JAM2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for JAM-B/VE-JAM Antibody (1G4) - Azide and BSA Free

• C21orf43
• CD322 antigen
• CD322
• JAM2
• JAMB
• JAM-B
• JAM-BJAM-IT/VE-JAM
• junctional adhesion molecule 2JAM-2
• junctional adhesion molecule B
• PRO245
• Vascular endothelial junction-associated molecule
• VE-JAM
• VE-JAMVEJAMCD322

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for JAM-B/VE-JAM Antibody (H00058494-M01)(1)

Publication using H00058494-M01

• Qi L, Liu J, Zhu H et al. Inhibition of Glioma Proliferation and Migration by Magnetic Nanoparticle Mediated JAM-2 silencing. J Mater Chem B 2020-04-09 [PMID: 32261795]

Bioinformatics

• Gene Symbol: JAM2
• Entrez:
  • 58494(Human)
• OMIM:
  • 606870(Human)
• Uniprot:
  • AAH17779(Human)