JAM-A Antibody (160407)

• Reactivity: Mu
• Applications: WB
• Clone: 160407
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

JAM-A Antibody (160407) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse JAM-A
  Lys27-Ala242
  Accession # O88792
• Specificity: Detects mouse JAM-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human JAM‑A.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: F11R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for JAM-A Antibody (160407)

• CD321 antigen
• CD321
• F11 receptor
• F11R
• JAM-1
• JAM1CD321
• JAMA
• JAM-A
• JCAMJAM
• Junctional adhesion molecule 1Platelet F11 receptor
• junctional adhesion molecule A
• PAM-1
• PAM-1KAT
• Platelet adhesion molecule 1

Background

The family of junctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-A is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (3). It is also expressed on circulating megakaryocytes. Mouse JAM-A cDNA predicts a 300 amino acid (aa) residue precursor protein with a putative 23 aa signal peptide, a 215 aa extracellular region containing two Ig-like V-subset domains, a 17 aa transmembrane domain and a 45 aa cytoplasmic domain. The human and mouse protein share approximately 67% aa sequence homology. Mouse JAM-A also shares approximately 35% aa sequence homology with mouse JAM-B or JAM-C. JAM-A exhibits homotypic interactions to regulate tight junction assembly and modulate paracellular permeability (1 - 3). The human JAM-A homotypic interation also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (4). JAM-A is involved in leukocyte adhesion and transmigration through the endothelium (3, 5). JAM-A has also been shown to bind reovirus attachment protein sigma-1 to permit reovirus infection and signal virus-induced apoptosis (6).

1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
3. Martin-Padura, I. et al. (1998) J. Cell Biol. 142:117.
4. Babinska, A. et al. (2002) Thromb. Haemost. 88:842.
5. Del Maschio, A. et al. (1999) J. Exp. Med. 190:1351.
6. Barton, E.S. et al. (2001) Cell 104:441.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: F11R
• Uniprot:
  • O88792()