Jagged 2 Antibody

Jagged 2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Jagged 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Jagged 2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Jagged 2
  Met27-Asp307
  Accession # Q9Y219
• Specificity: Detects human Jagged 2 in direct ELISAs and Western blots. In Western blots, approximately 35% cross-reactivity with recombinant human Jagged 1 is observed and less than 1% cross-reactivity with recombinant rat Jagged 1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: JAG2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jagged 2 Antibody

• HJ2
• JAG2
• Jagged 2
• Jagged2
• protein jagged-2
• SER2

Background

Human Jagged 2 is a 131 kDa (predicted) member of the Delta-Serrate-Lag-2 (DSL) family of ligands. This family activates LIN12/Notch proteins and thereby regulates cell fate determination during development (1-5). It is a type 1 transmembrane protein that is synthesized as a 1238 amino acid (aa) precursor. It contains a 23 aa signal sequence, a large 1057 aa extracellular region, a 21 aa transmembrane region, and a short 137 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), a von Willebrand factor (vWF) type C domain, and a cysteine-rich region just proximal to the transmembrane segment (2). There are two isoforms for human Jagged 2, named long and short. The short form lacks a splicing variant region (aa 421-461) that is present in the long form of the protein. Human Jagged 2 shares 90% and 87% aa sequence identity with mouse and rat Jagged 2, respectively. During murine embryonic development, Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (2). In 2 week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (2). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (2). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, T cell lineage differentiation, natural killer cells, and the establishment of functional natural killer cell lines (3, 5, 6).

1. Shawber, C. et al. (1996) Dev. Biol. 180:370.
2. Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
3. Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
4. Schickwann, T. et al. (2000) Blood 96:950.
5. DeHart, S. et al. (2005) Blood 105:3521.
6. de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Jagged 2 Antibody (AF1726)(2)

Publications using AF1726

• Xing F, Okuda H, Watabe M, Kobayashi A, Pai SK, Liu W, Pandey PR, Fukuda K, Hirota S, Sugai T, Wakabayshi G, Koeda K, Kashiwaba M, Suzuki K, Chiba T, Endo M, Mo YY, Watabe K Hypoxia-induced Jagged2 promotes breast cancer metastasis and self-renewal of cancer stem-like cells. Oncogene, 2011-04-18;30(39):4075-86. 2011-04-18 [PMID: 21499308] (ICC, IHC-P, Western Blot, Human)
• Uenotsuchi T, Takeuchi S, Matsuda T, Urabe K, Koga T, Uchi H, Fukagawa S, Kawasaki M, Kajiwara H, Moroi Y, Furue M Differential induction of Th1-prone immunity by human dendritic cells activated with Sporothrix schenckii of cutaneous and visceral origins to determine their different virulence. Int. Immunol., 2006-10-11;18(12):1637-46. 2006-10-11 [PMID: 17035348] (Western Blot, Human)

Bioinformatics

• Gene Symbol: JAG2
• Uniprot:
  • Q9Y219(Human)