Jagged 1 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Jagged 1 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived rhJagged 1
  Ser32-Asp296
  Accession # P78504
• Specificity: Detects human Jagged 1 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant rat Jagged 1 is observed, and 10% cross-reactivity with recombinant human Jagged 2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: JAG1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jagged 1 Antibody [Biotin]

• AGS
• AHDMGC104644
• Alagille syndrome
• AWS
• CD339 antigen
• CD339
• HJ1
• JAG1
• Jagged 1
• Jagged1
• JAGL1
• protein jagged-1

Background

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
4. Lai, E.C. (2004) Development 131:965.
5. Milner, L.A. et al. (1994) Blood 83:2057.  
6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504.
7. Linheng, L. et al. (1998) Immunity 8:43.
8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
9. Oda, T. et al. (1997) Nat. Genet. 16:235.
10. Oda, T. et al. (1997) Genomics 43:376.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Jagged 1 Antibody (BAF1277)(1)

Publication using BAF1277

• Deep Shah, Debra Wyatt, Andrew T. Baker, Patricia Simms, Daniel S. Peiffer, Michelle Fernandez et al. Inhibition of HER2 Increases JAGGED1-dependent Breast Cancer Stem Cells: Role for Membrane JAGGED1 Clinical Cancer Research 2018-09-15 [PMID: 29895705]

Bioinformatics

• Gene Symbol: JAG1
• Uniprot:
  • P78504(Human)