Jagged 1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of Huh‑7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal ...read more
Recombinant Human Jagged 1 Fc Chimera (Catalog # 1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (Catalog # ...read more
Jagged 1 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) at 15 µg/mL overnight at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Jagged 1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Jagged 1
Ser32-Asp296
Accession # P78504
Specificity
Detects human Jagged 1 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant rat Jagged 1 and recombinant human Jagged 2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
JAG1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 1-5 ug/mL
Control
Jagged 1 Knockout HeLa Cell Lysate
Publications
Read Publications using
AF1277 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jagged 1 Antibody [Unconjugated]

  • AGS
  • AHDMGC104644
  • Alagille syndrome
  • AWS
  • CD339 antigen
  • CD339
  • HJ1
  • JAG1
  • Jagged 1
  • Jagged1
  • JAGL1
  • protein jagged-1

Background

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1‑8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

  1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
  2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
  3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
  4. Lai, E.C. (2004) Development 131:965.
  5. Milner, L.A. et al. (1994) Blood 83:2057.  
  6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504.
  7. Linheng, L. et al. (1998) Immunity 8:43.
  8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
  9. Oda, T. et al. (1997) Nat. Genet. 16:235.
  10. Oda, T. et al. (1997) Genomics 43:376.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Jagged 1 Antibody (AF1277)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Bioassay, ICC, IHC-P, Neutralization, Western Blot.


Filter By Application
Bioassay
(1)
ICC
(2)
IHC-P
(1)
Neutralization
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF1277 Applications Species
F De Falco, B Del Papa, S Baldoni, R Sabatini, F Falzetti, M Di Ianni, MP Martelli, F Mezzasoma, M Pelullo, P Marconi, P Sportolett, I Screpanti, E Rosati IL-4-dependent Jagged1 expression/processing is associated with survival of chronic lymphocytic leukemia cells but not with Notch activation Cell Death Dis, 2018;9(12):1160. 2018 [PMID: 30478302] (Neutralization, Human) Neutralization Human
M Bellon, R Moles, H Chaib-Mezr, J Pancewicz, C Nicot JAG1 overexpression contributes to Notch1 signaling and the migration of HTLV-1-transformed ATL cells J Hematol Oncol, 2018;11(1):119. 2018 [PMID: 30231940] (ICC, Human) ICC Human
P Sansone, M Berishaj, VK Rajasekhar, C Ceccarelli, Q Chang, A Strillacci, C Savini, L Shapiro, R Bowman, C Mastroleo, S De Carolis, L Daly, A Benito-Mar, F Perna, N Fabbri, JH Healey, E Spisni, M Cricca, D Lyden, M Bonafé, J Bromberg Evolution of cancer stem-like cells in endocrine-resistant metastatic breast cancers is mediated by stromal microvesicles Cancer Res, 2017;0(0):. 2017 [PMID: 28202520] (Bioassay, Human) Bioassay Human
Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala-Dilke K, Leinster D, Zhong H, Hollingsworth R, Thompson R, Whiteford J, Balkwill F Interleukin-6 Stimulates Defective Angiogenesis. Cancer Res, 2015;75(15):3098-107. 2015 [PMID: 26081809] (IHC-P, Human) IHC-P Human
Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment. Cancer Res., 2012;72(1):66-75. 2012 [PMID: 22065722] (Western Blot, Human) Western Blot Human
Coward J, Kulbe H, Chakravarty P, Leader D, Vassileva V, Leinster DA, Thompson R, Schioppa T, Nemeth J, Vermeulen J, Singh N, Avril N, Cummings J, Rexhepaj E, Jirstrom K, Gallagher WM, Brennan DJ, McNeish IA, Balkwill FR Interleukin-6 as a therapeutic target in human ovarian cancer. Clin. Cancer Res., 2011;17(18):6083-96. 2011 [PMID: 21795409] (Western Blot, Human) Western Blot Human
Roesli C, Mumprecht V, Neri D, Detmar M Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping. FASEB J., 2008;22(6):1933-44. 2008 [PMID: 18180333] (Western Blot, Human) Western Blot Human
Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling. Stem Cells, 2007;26(1):279-89. 2007 [PMID: 17962701] (ICC, Human) ICC Human

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1277

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Bioinformatics

Gene Symbol JAG1
Uniprot