Jagged 1 Antibody [Unconjugated]

Western blot shows lysates of Huh-7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Jagged 1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Jagged 1 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Recombinant Human Jagged 1 Fc Chimera (Catalog # 1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Jagged 1 Antibody (Catalog # AF1277). The ND50 is typically 1-5 µg/mL.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Jagged 1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Jagged 1
  Ser32-Asp296
  Accession # P78504
• Specificity: Detects human Jagged 1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: JAG1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 1-5 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Jagged 1 Antibody [Unconjugated]

• AGS
• AHDMGC104644
• Alagille syndrome
• AWS
• CD339 antigen
• CD339
• HJ1
• JAG1
• Jagged 1
• Jagged1
• JAGL1
• protein jagged-1

Background

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
4. Lai, E.C. (2004) Development 131:965.
5. Milner, L.A. et al. (1994) Blood 83:2057.  
6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504.
7. Linheng, L. et al. (1998) Immunity 8:43.
8. Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
9. Oda, T. et al. (1997) Nat. Genet. 16:235.
10. Oda, T. et al. (1997) Genomics 43:376.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Jagged 1 Antibody (AF1277)(27)

Publications using AF1277

• Mikuli?, P;Ogorevc, M;Petri?evi?, M;Kali?anin, D;Tafra, R;Saraga-Babi?, M;Mardeši?, S; SOX2, JAGGED1, ?-Catenin, and Vitamin D Receptor Expression Patterns during Early Development and Innervation of the Human Inner Ear International journal of molecular sciences 2024-08-09 [PMID: 39201406] (Immunohistochemistry, Human)
• Herrera, JL;Komatsu, M; Akt3 activation by R-Ras in an endothelial cell enforces quiescence and barrier stability of neighboring endothelial cells via Jagged1 Cell reports 2024-02-24 [PMID: 38402584] (Neutralization, Human)
• G Wang, B Heijs, S Kostidis, RGJ Rietjens, M Koning, L Yuan, GL Tiemeier, A Mahfouz, SJ Dumas, M Giera, J Kers, SM Chuva de S, CW van den Be, BM van den Be, TJ Rabelink Spatial dynamic metabolomics identifies metabolic cell fate trajectories in human kidney differentiation Cell Stem Cell, 2022-11-03;29(11):1580-1593.e7. 2022-11-03 [PMID: 36332571] (IHC, Human)
• Lin Zhao, Jianjun Lei, Shanzhi Gu, Yujiao Zhang, Xin Jing, Lu Wang et al. A yes-associated protein 1- Notch1 receptor positive feedback loop promotes breast cancer lung metastasis by attenuating the bone morphogenetic protein 4-SMAD family member 1/5 signaling Carcinogenesis 12/31/2022 [PMID: 36194598]
• Lin Zhao, Jianjun Lei, Shanzhi Gu, Yujiao Zhang, Xin Jing, Lu Wang et al. A yes-associated protein 1- Notch1 receptor positive feedback loop promotes breast cancer lung metastasis by attenuating the bone morphogenetic protein 4-SMAD family member 1/5 signaling Carcinogenesis 2022-12-31 [PMID: 36194598] (Bioassay, Human)
• ET Parkin, JE Hammond, L Owens, MD Hodges The orphan drug dichloroacetate reduces amyloid beta-peptide production whilst promoting non-amyloidogenic proteolysis of the amyloid precursor protein PLoS ONE, 2022-01-13;17(1):e0255715. 1/13/2022 [PMID: 35025874]
• T Iosifidis, EN Sutanto, ST Montgomery, P Agudelo-Ro, K Looi, KM Ling, NC Shaw, LW Garratt, J Hillas, KM Martinovic, E Kicic-Star, S Vijayaseka, FJ Lannigan, PJ Rigby, DA Knight, SM Stick, A Kicic Dysregulated Notch Signaling in the Airway Epithelium of Children with Wheeze Journal of personalized medicine, 2021-12-07;11(12):. 2021-12-07 [PMID: 34945795] (In-Cell Western (ICW), Human)
• Pierfrancesco Pagella, Laura de Vargas Roditi, Bernd Stadlinger, Andreas E. Moor, Thimios A. Mitsiadis Notch signaling in the dynamics of perivascular stem cells and their niches Stem Cells Transl Med 7/6/2021 [PMID: 34227747]
• Junjie Yu, Changyu Zhu, Xiaobo Wang, KyeongJin Kim, Alberto Bartolome, Paola Dongiovanni et al. Hepatocyte TLR4 triggers inter-hepatocyte Jagged1/Notch signaling to determine NASH-induced fibrosis Science Translational Medicine 6/23/2021 [PMID: 34162749]
• MT Palano, D Giannandre, N Platonova, G Gaudenzi, M Falleni, D Tosi, E Lesma, V Citro, M Colombo, I Saltarella, R Ria, N Amodio, E Taiana, A Neri, G Vitale, R Chiaramont Jagged Ligands Enhance the Pro-Angiogenic Activity of Multiple Myeloma Cells Cancers (Basel), 2020-09-11;12(9):. 2020-09-11 [PMID: 32932949] (IHC, Human)
• Bersini S, Schulte R, Huang L et al. Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome eLife 9/8/2020 [PMID: 32896271]
• JH Low, P Li, EGY Chew, B Zhou, K Suzuki, T Zhang, MM Lian, M Liu, E Aizawa, C Rodriguez, KSM Yong, Q Chen, JM Campistol, M Fang, CC Khor, JN Foo, JC Izpisua Be, Y Xia Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network Cell Stem Cell, 2019-07-11;0(0):. 7/11/2019 [PMID: 31303547]
• F De Falco, B Del Papa, S Baldoni, R Sabatini, F Falzetti, M Di Ianni, MP Martelli, F Mezzasoma, M Pelullo, P Marconi, P Sportolett, I Screpanti, E Rosati IL-4-dependent Jagged1 expression/processing is associated with survival of chronic lymphocytic leukemia cells but not with Notch activation Cell Death Dis, 2018-11-26;9(12):1160. 2018-11-26 [PMID: 30478302] (Neutralization, Human)
• M Bellon, R Moles, H Chaib-Mezr, J Pancewicz, C Nicot JAG1 overexpression contributes to Notch1 signaling and the migration of HTLV-1-transformed ATL cells J Hematol Oncol, 2018-09-19;11(1):119. 2018-09-19 [PMID: 30231940] (ICC, Human)
• P Sansone, M Berishaj, VK Rajasekhar, C Ceccarelli, Q Chang, A Strillacci, C Savini, L Shapiro, R Bowman, C Mastroleo, S De Carolis, L Daly, A Benito-Mar, F Perna, N Fabbri, JH Healey, E Spisni, M Cricca, D Lyden, M Bonafé, J Bromberg Evolution of cancer stem-like cells in endocrine-resistant metastatic breast cancers is mediated by stromal microvesicles Cancer Res, 2017-02-15;0(0):. 2017-02-15 [PMID: 28202520] (Bioassay, Human)
• Wantae Kim, Sanjoy Kumar Khan, Jelena Gvozdenovic-Jeremic, Youngeun Kim, Jason Dahlman, Hanjun Kim et al. Hippo signaling interactions with Wnt/ beta -catenin and Notch signaling repress liver tumorigenesis Journal of Clinical Investigation 11/21/2016 [PMID: 27869648]
• Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala-Dilke K, Leinster D, Zhong H, Hollingsworth R, Thompson R, Whiteford J, Balkwill F Interleukin-6 Stimulates Defective Angiogenesis. Cancer Res, 2015-06-16;75(15):3098-107. 2015-06-16 [PMID: 26081809] (IHC-P, Human)
• S Sahebjam, P L Bedard, V Castonguay, Z Chen, M Reedijk, G Liu et al. A phase I study of the combination of ro4929097 and cediranib in patients with advanced solid tumours (PJC-004/NCI 8503) British Journal of Cancer 2013-08-20 [PMID: 23868004] (Immunohistochemistry, Human)
• S Sahebjam, P L Bedard, V Castonguay, Z Chen, M Reedijk, G Liu et al. A phase I study of the combination of ro4929097 and cediranib in patients with advanced solid tumours (PJC-004/NCI 8503) British Journal of Cancer 8/20/2013 [PMID: 23868004]
• Ivan Diaz-Padilla, Hal Hirte, Amit M. Oza, Blaise A. Clarke, Brenda Cohen, Michael Reedjik et al. A phase Ib combination study of RO4929097, a gamma-secretase inhibitor, and temsirolimus in patients with advanced solid tumors Investigational New Drugs 10/1/2013 [PMID: 23860641]
• Ivan Diaz-Padilla, Hal Hirte, Amit M. Oza, Blaise A. Clarke, Brenda Cohen, Michael Reedjik et al. A phase Ib combination study of RO4929097, a gamma-secretase inhibitor, and temsirolimus in patients with advanced solid tumors Investigational New Drugs 2013-10-01 [PMID: 23860641] (Immunohistochemistry, Human)
• Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment. Cancer Res., 2011-11-07;72(1):66-75. 2011-11-07 [PMID: 22065722] (Western Blot, Human)
• Coward J, Kulbe H, Chakravarty P, Leader D, Vassileva V, Leinster DA, Thompson R, Schioppa T, Nemeth J, Vermeulen J, Singh N, Avril N, Cummings J, Rexhepaj E, Jirstrom K, Gallagher WM, Brennan DJ, McNeish IA, Balkwill FR Interleukin-6 as a therapeutic target in human ovarian cancer. Clin. Cancer Res., 2011-07-27;17(18):6083-96. 2011-07-27 [PMID: 21795409] (IHC-P, Western Blot, Human)
• Nibal Zaghloul, Sonia L. Hernandez, Jae-O Bae, Jianzhong Huang, Jason C Fisher, Alice Lee et al. Vascular endothelial growth factor blockade rapidly elicits alternative proangiogenic pathways in neuroblastoma Int J Oncol 3/24/2011 [PMID: 19148474]
• Nibal Zaghloul, Sonia L. Hernandez, Jae-O Bae, Jianzhong Huang, Jason C Fisher, Alice Lee et al. Vascular endothelial growth factor blockade rapidly elicits alternative proangiogenic pathways in neuroblastoma Int J Oncol 2011-03-24 [PMID: 19148474] (Immunohistochemistry, Mouse)
• Roesli C, Mumprecht V, Neri D, Detmar M Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping. FASEB J., 2008-01-07;22(6):1933-44. 2008-01-07 [PMID: 18180333] (Western Blot, Human)
• Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling. Stem Cells, 2007-10-25;26(1):279-89. 2007-10-25 [PMID: 17962701] (ICC, Human)

Bioinformatics

• Gene Symbol: JAG1
• Uniprot:
  • P78504(Human)