JAB1 Antibody (OTI2B12) - Azide and BSA Free

Western Blot: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY COPS5 (RC200979, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti COPS

Immunocytochemistry/Immunofluorescence: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY COPS5(RC200979).

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Carcinoma of Human kidney tissue. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using antiCOPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Human bladder tissue within the normal limits using antiCOPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Human Ovary tissue within the normal limits using antiCOPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-COPS5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

Immunohistochemistry: JAB1 Antibody (OTI2B12) - Azide and BSA Free [NBP2-71659] - Staining of paraffin-embedded Human Kidney tissue within the normal limits. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI2B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

JAB1 Antibody (OTI2B12) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse JAB1 Antibody (OTI2B12) - Azide and BSA Free (NBP2-01813) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human COPS5(NP_006828) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: COPS5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 37.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for JAB1 Antibody (OTI2B12) - Azide and BSA Free

• COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis)
• COP9 signalosome complex subunit 5
• COPS5
• CSN5
• CSN5COP9 (constitutive photomorphogenic, Arabidopsis, homolog) subunit 5
• EC 3.4
• JAB1
• JAB138 kDa Mov34 homolog
• Jun activation domain-binding protein 1
• MOV-34
• SGN5
• SGN5MGC3149
• Signalosome subunit 5

Background

The protein encoded by the JAB1 gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COPS5