IVD Antibody (OTI1B10) - Azide and BSA Free

Western Blot: IVD Antibody (OTI1B10) - Azide and BSA Free [NBP2-71055] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IVD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IVD.

Immunocytochemistry/Immunofluorescence: IVD Antibody (OTI1B10) - Azide and BSA Free [NBP2-71055] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IVD.

Immunohistochemistry: IVD Antibody (OTI1B10) - Azide and BSA Free [NBP2-71055] - Staining of paraffin-embedded Human lymph node tissue using anti-IVD mouse monoclonal antibody.

Western Blot: IVD Antibody (OTI1B10) - Azide and BSA Free [NBP2-71055] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IVD monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

IVD Antibody (OTI1B10) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human IVD (NP_002612) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IVD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Theoretical MW: 46.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for IVD Antibody (OTI1B10) - Azide and BSA Free

• ACAD2
• EC 1.3.99
• EC 1.3.99.10
• FLJ12715
• FLJ34849
• isovaleryl-CoA dehydrogenase
• mitochondrial

Background

Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IVD