ITM2B Antibody (OTI1C11)

Western Blot: ITM2B Antibody (1C11) [NBP2-00620] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ITM2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ITM2B.

Immunohistochemistry-Paraffin: ITM2B Antibody (1C11) [NBP2-00620] Staining of paraffin-embedded Human Kidney tissue using anti-ITM2B mouse monoclonal antibody.

Flow Cytometry: ITM2B Antibody (1C11) [NBP2-00620] - Analysis of Jurkat cells, using anti-ITM2B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: ITM2B Antibody (1C11) [NBP2-00620] - Analysis of Hela cells, using anti-ITM2B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI1C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

ITM2B Antibody (OTI1C11) Summary

• Description: Novus Biologicals Mouse ITM2B Antibody (OTI1C11) (NBP2-00620) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human ITM2B (NP_068839) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITM2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 30.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ITM2B Antibody (OTI1C11)

• ABRI
• ABri/ADan amyloid peptide
• BRI
• BRI2
• BRICD2B
• BRICHOS domain containing 2B
• BRIFBD
• E25B
• E3-16
• FBD
• integral membrane protein 2B
• ITM2B
• Protein E25B
• Transmembrane protein BRI

Background

Amyloid precursor proteins are processed by beta-secretase and gamma-secretase to produce beta-amyloid peptides which form the characteristic plaques of Alzheimer disease. This gene encodes a transmembrane protein which is processed at the C-terminus by furin or furin-like proteases to produce a small secreted peptide which inhibits the deposition of beta-amyloid. Mutations which result in extension of the C-terminal end of the encoded protein, thereby increasing the size of the secreted peptide, are associated with two neurogenerative diseases, familial British dementia and familial Danish dementia. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITM2B
• Entrez:
  • 9445(Human)