| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88354-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71460 | |||
CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and plaques. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="ITM2B was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Sheep Anti-Human ITM2B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7015) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and plaques. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7015-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57975-25ul | |||
![Western Blot: ITM2B Antibody [NBP1-36963] - HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ITM2B-Antibody-Western-Blot-NBP1-36963-img0006.jpg "Western Blot: ITM2B Antibody [NBP1-36963] - HepG2 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: ITM2B Antibody [NBP1-36963] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi apparatus and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10u g/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/ITM2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-36963-img0008.jpg)
![Immunohistochemistry-Paraffin: ITM2B Antibody [NBP1-36963] - Human small intestine tissue. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ITM2B-Antibody-Immunohistochemistry-Paraffin-NBP1-36963-img0003.jpg)
![Flow Cytometry: ITM2B Antibody [NBP1-36963] - Paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/ITM2B-Antibody-Flow-Cytometry-NBP1-36963-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ITM2B Antibody [NBP1-36963] - Paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi apparatus and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/ITM2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-36963-img0007.jpg)
![Immunohistochemistry: ITM2B Antibody [NBP2-17000] - ITM2B antibody detects ITM2B protein at Golgi apparatus on mouse esophagus by immunohistochemical analysis. <BR/>Sample: Paraffin-embedded mouse brain stem. <BR/>ITM2B antibody (NBP2-17000) dilution: 1:500.<BR/><br>Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp2-17000_rabbit-polyclonal-itm2b-antibody-9102024205619.jpg "Immunohistochemistry: ITM2B Antibody [NBP2-17000] - ITM2B antibody detects ITM2B protein at Golgi apparatus on mouse esophagus by immunohistochemical analysis. <BR/>Sample: Paraffin-embedded mouse brain stem. <BR/>ITM2B antibody (NBP2-17000) dilution: 1:500.<BR/><br>Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Western Blot: ITM2B Antibody [NBP2-17000] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with ITM2B antibody (NBP2-17000) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17000_rabbit-polyclonal-itm2b-antibody-91020242014623.jpg)
![Immunocytochemistry/Immunofluorescence: ITM2B Antibody [NBP1-88354] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ITM2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88354-img0008.jpg "Immunocytochemistry/Immunofluorescence: ITM2B Antibody [NBP1-88354] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles. Antibody staining is shown in green.")
![Western Blot: ITM2B Antibody (1C11) [NBP2-00620] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ITM2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ITM2B.](http://images.novusbio.com/fullsize/ITM2B-Antibody-1C11-Western-Blot-NBP2-00620-img0003.jpg "Western Blot: ITM2B Antibody (1C11) [NBP2-00620] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ITM2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ITM2B.")
![Immunohistochemistry-Paraffin: ITM2B Antibody (1C11) [NBP2-00620] Staining of paraffin-embedded Human Kidney tissue using anti-ITM2B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ITM2B-Antibody-1C11-Immunohistochemistry-Paraffin-NBP2-00620-img0004.jpg)
![Flow Cytometry: ITM2B Antibody (1C11) [NBP2-00620] - Analysis of Jurkat cells, using anti-ITM2B antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ITM2B-Antibody-1C11-Flow-Cytometry-NBP2-00620-img0002.jpg)
![Flow Cytometry: ITM2B Antibody (1C11) [NBP2-00620] - Analysis of Hela cells, using anti-ITM2B antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ITM2B-Antibody-1C11-Flow-Cytometry-NBP2-00620-img0001.jpg)
![SDS-Page: Recombinant Human ITM2B Protein [H00009445-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00009445-P02-1.jpg "SDS-Page: Recombinant Human ITM2B Protein [H00009445-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: ITM2B Antibody [NBP2-57975] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/ITM2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57975-img0001.jpg "Immunocytochemistry/Immunofluorescence: ITM2B Antibody [NBP2-57975] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus.")
![Western Blot: ITM2B Overexpression Lysate (Adult Normal) [NBL1-12082] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ITM2B.](http://images.novusbio.com/fullsize/ITM2B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12082-img0002.jpg "Western Blot: ITM2B Overexpression Lysate (Adult Normal) [NBL1-12082] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ITM2B.")
![ELISA: ITM2B Antibody (1A10) [H00009445-M05] - Detection limit for recombinant GST tagged ITM2B is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ITM2B-Antibody-1A10-ELISA-H00009445-M05-img0001.jpg "ELISA: ITM2B Antibody (1A10) [H00009445-M05] - Detection limit for recombinant GST tagged ITM2B is approximately 1ng/ml as a capture antibody.")
