ITFG1 Antibody (936234) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, human brain (cerebellum) tissue, and human testis tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ITFG1 Monoclonal Antibody (Catalog # MAB8900) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ITFG1 at approximately 95-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 936234
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ITFG1 Antibody (936234) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived human ITFG1
  Met1-Ile566
  Accession # Q8TB96
• Specificity: Detects human ITFG1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITFG1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ITFG1 Antibody (936234) [Unconjugated]

• CDA08
• integrin alpha FG-GAP repeat containing 1,2310047C21Rik
• Integrin-alpha FG-GAP repeat-containing protein 1
• ITFG1
• Protein TIP
• T-cell immunomodulatory protein
• TIP
• TIPT cell immunomodulatory protein

Background

Integrin-alpha FG-GAP repeat-containing protein 1 (ITFG1), also known as T cell immunomodulatory protein (TIP), was initially identified using bioinformatics and high-throughput cell-based screening assays to isolate novel factors involved in T cell biology.  A ubiquitously expressed 98 kDa glycoprotein, ITFG1 contains an N-terminal signal peptide and a C-terminal transmembrane domain flanking twelve potential N-linked glycosylation sites.  Human and mouse T cells treated with ITFG1 in vitro secreted the cytokines IFN-g, TNF-a and IL-10, while in vivo ITFG1 was protective in a mouse acute graft-versus-host disease (GVHD) model.  Over amino acids (aa) 1-566, human ITFG1 shares 89% and 88% aa identity with mouse and rat ITFG1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITFG1
• Uniprot:
  • Q8TB96()