Isocitrate Dehydrogenase 2/IDH2 Antibody (1069032) [Unconjugated]

Jurkat cells were stained with Mouse Anti-Human Isocitrate Dehydrogenase 2/IDH2 Monoclonal Antibody (Catalog # MAB11462, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Isocitrate Dehydrogenase 2/IDH2 was detected in fixed MCF‑7 human breast cancer cell line (Positive) and SK‑Mel‑28 human malignant melanoma cell line (Positive) using Mouse Anti-Human Isocitrate Dehydrogenase 2/IDH2 Monoclonal Antibody (Catalog # MAB11462) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Isocitrate Dehydrogenase 2/IDH2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Isocitrate Dehydrogenase 2/IDH2 Monoclonal Antibody (Catalog # MAB11462) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and kidney tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC, Flow, ICC/IF
• Clone: 1069032
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Isocitrate Dehydrogenase 2/IDH2 Antibody (1069032) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IDH-2
  Thr376-Gln452
  Accession # P48735
• Specificity: Detects human IDH-2 in direct ELISA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 3-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Isocitrate Dehydrogenase 2/IDH2 Antibody (1069032) [Unconjugated]

• D2HGA2
• ICD-M
• IDH2
• IDHM
• IDPM
• Isocitrate Dehydrogenase 2

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P48735(Human)