Islet-1 Overexpression Lysate (Native)

Images

 
Western Blot: Islet 1 Overexpression Lysate (Adult Normal) [NBL1-12050] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Islet 1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Islet-1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206893 and based on accession number NM_002202. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens ISL LIM homeobox 1 (ISL1), mRNA.
Gene
ISL1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
38.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Islet-1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Islet-1 Overexpression Lysate (Native)

  • insulin gene enhancer protein ISL-1
  • ISL LIM homeobox 1
  • ISL1 transcription factor, LIM/homeodomain
  • ISL1 transcription factor, LIM/homeodomain, (islet-1)
  • ISL1
  • Isl-1
  • Islet1
  • Islet-1

Background

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Islet-1 Lysate (NBL1-12050) (0)

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol ISL1
Entrez