Islet-1 Antibody (8A3)

Immunocytochemistry/Immunofluorescence: Islet-1 Antibody (8A3) [H00003670-M03] - Analysis of monoclonal antibody to ISL1 on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: Islet-1 Antibody (8A3) [H00003670-M03] - Detection limit for recombinant GST tagged ISL1 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, S-ELISA
• Clone: 8A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Islet-1 Antibody (8A3) Summary

• Immunogen: ISL1 (NP_002193, 63 a.a. ~ 159 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEP
• Localization: Nuclear, occasionally cytoplasmic
• Specificity: ISL1 - ISL1 transcription factor, LIM/homeodomain, (islet-1)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ISL1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Islet-1 Antibody (8A3)

• insulin gene enhancer protein ISL-1
• ISL LIM homeobox 1
• ISL1 transcription factor, LIM/homeodomain
• ISL1 transcription factor, LIM/homeodomain, (islet-1)
• ISL1
• Isl-1
• Islet1
• Islet-1

Background

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ISL1
• Entrez:
  • 3670(Human)
• OMIM:
  • 600366(Human)
• Uniprot:
  • NP_002193(Human)