Islet-1 Antibody (1A3)

Images

 
Western Blot: Islet-1 Antibody (1A3) [H00003670-M01] - ISL1 monoclonal antibody (M01), clone 1A3. Analysis of ISL1 expression in K-562.
Western Blot: Islet-1 Antibody (1A3) [H00003670-M01] - Analysis of ISL1 over-expressed 293 cell line, cotransfected with ISL1 Validated Chimera RNAi ( Cat # H00003670-R01V ) (Lane 2) or non-transfected control (Lane 1). ...read more
Western Blot: Islet-1 Antibody (1A3) [H00003670-M01] - ISL1 monoclonal antibody (M01), clone 1A3. Analysis of ISL1 expression in Hela S3 NE.
Western Blot: Islet-1 Antibody (1A3) [H00003670-M01] - Analysis of ISL1 expression in transfected 293T cell line by ISL1 monoclonal antibody (M01), clone 1A3.Lane 1: ISL1 transfected lysate(38.7 KDa).Lane 2: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, RNAi
Clone
1A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Islet-1 Antibody (1A3) Summary

Immunogen
ISL1 (NP_002193 63 a.a. - 159 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YCKRDYIRLYGIKCAKCSIGFSKNDFVMRARSKVYHIECFRCVACSRQLIPGDEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAEP
Localization
Nuclear, occasionally cytoplasmic
Specificity
ISL1 - ISL1 transcription factor, LIM/homeodomain, (islet-1)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ISL1
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • RNA Inhibition
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Ascites

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Islet-1 Antibody (1A3)

  • insulin gene enhancer protein ISL-1
  • ISL LIM homeobox 1
  • ISL1 transcription factor, LIM/homeodomain
  • ISL1 transcription factor, LIM/homeodomain, (islet-1)
  • ISL1
  • Isl-1
  • Islet1
  • Islet-1

Background

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ISL1
Entrez
OMIM
Uniprot