IRP2 Antibody (9D1-R)

Western Blot: IRP2 Antibody (9D1-R) [NBP3-32483] - Western blot analysis of IRP2 on different lysates with Mouse anti-IRP2 antibody (NBP3-32483) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: Mouse liver tissue lysate (40 ug/Lane) Lane 4: Mouse kidney tissue lysate (40 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32483) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 9D1-R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IRP2 Antibody (9D1-R) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human IRP2 aa 1-963. (Uniprot: P48200)
• Localization: Cytoplasm.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IREB2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 105 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IRP2 Antibody (9D1-R)

• ACO3
• EC 4.2.1.3
• FLJ23381
• IRE-BP 2
• Iron regulatory protein 2
• iron-responsive element binding protein 2
• iron-responsive element-binding protein 2
• IRP2IRP2AD

Background

Iron regulatory protein 2 (IRP2) is a central post-transcriptional regulator of cellular and systemic iron metabolism. It is a cytosolic RNA-binding protein that binds to stem-loop structures. IRP2 is regulated by iron-mediated proteolysis. IRP2 also plays a role during hypoxia, as iron and oxygen are closely related.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IREB2