IRGM Antibody

Images

 
Western Blot: IRGM Antibody [NBP1-76890] - Analysis in human brain lysate with antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: IRGM Antibody [NBP1-76890] - of IRGM in Human Brain cells. Dilution: 20 ug/mL.
Immunohistochemistry-Paraffin: IRGM Antibody [NBP1-76890] - Human brain with IRGM antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

IRGM Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the center of the human IRGM. The immunogen is located within the last 50 amino acids of IRGM.
Specificity
IRGM antibody may recognize mouse IRGM2 and IRGM3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IRGM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
IRGM Peptide (NBP1-76890PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IRGM Antibody

  • IFI1
  • immunity-related GTPase family M protein
  • immunity-related GTPase family, M
  • interferon-inducible protein 1
  • IRGM1
  • LPS-stimulated RAW 264.7 macrophage protein 47 homolog
  • LRG47
  • LRG-47
  • LRG-47-like protein
  • M1
  • MGC149263
  • MGC149264

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn's disease. In mouse, IRGM belongs to a family of gamma-interferon-induced GTP-binding proteins of approximately 48 kDa. Murine IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. Human IRGM is also involved in autophagy and plays a role in the control of intracellular pathogens and in the reduction of intracellular bacillary load.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IRGM
Uniprot