IRF9 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: IRF9 Antibody [NBP1-81577] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: IRF9 Antibody [NBP1-81577] - Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IRF9 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IRF9 Antibody - BSA Free (NBP1-81577) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-IRF9 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: EEEDAMQNCTLSPSVLQDSLNNEEEGASGGAVHSDIGSSSSSSSPEPQEVTDTTEAPFQGDQRSLEFLLPPEPDYSLLLTFIYNGRVVGEAQVQSLDCRLVAEPSGSESSMEQVLFPKPGPLEPTQRLLSQLER
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRF9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  IRF9 Protein (NBP1-81577PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for IRF9 Antibody - BSA Free

• IFN-alpha-responsive transcription factor subunit
• interferon regulatory factor 9
• Interferon-stimulated gene factor 3 gamma
• interferon-stimulated transcription factor 3, gamma (48kD)
• interferon-stimulated transcription factor 3, gamma 48kDa
• IRF9
• IRF-9
• ISGF3 p48 subunit
• ISGF3
• ISGF3G
• ISGF3GISGF-3 gamma
• p48
• Transcriptional regulator ISGF3 subunit gamma

Background

Transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRF9 Antibody (NBP1-81577)(1)

Publication using NBP1-81577

• Drury S, Clauson G, Zetterman A et al. Evolution and Emergence of Primate Specific Interferon Regulatory Factor 9 Journal of medical virology 2023-01-24 [PMID: 36691924] (WB, Human)

Bioinformatics

• Gene Symbol: IRF9