IRF4 Antibody (CL14615) - Azide and BSA Free

Analysis in human tonsil and skeletal muscle tissues using NBP3-44146 antibody. Corresponding IRF4 RNA-seq data are presented for the same tissues.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human small intestine shows strong nuclear positivity in lymphoid cells.

Analysis in human cell line SK-MEL-30.

Staining of human tonsil shows strong nuclear positivity in lymphoid cells.

Staining of human skin shows strong nuclear positivity in a subset of lymphoid cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL14615
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

IRF4 Antibody (CL14615) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse IRF4 Antibody (CL14615) - Azide and BSA Free (NBP3-26863) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q15306, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRF4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for IRF4 Antibody (CL14615) - Azide and BSA Free

• interferon regulatory factor 4
• IRF4
• IRF-4
• LSIRF
• LSIRFMUM1
• Lymphocyte-specific interferon regulatory factor
• Multiple myeloma oncogene 1
• MUM1
• NF-EM5

Background

Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRF4