IRAK1 Antibody (3F7)

Western Blot: IRAK1 Antibody (3F7) [H00003654-M01] - Analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M01), clone 3F7.Lane 1: IRAK1 transfected lysate(68 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: IRAK1 Antibody (3F7) [H00003654-M01] - Analysis of monoclonal antibody to IRAK1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: IRAK1 Antibody (3F7) [H00003654-M01] - Analysis of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Western Blot: IRAK1 Antibody (3F7) [H00003654-M01] - Western Blot analysis of IRAK1 expression in K-562 ( Cat # L009V1 ).

Immunoprecipitation: IRAK1 Antibody (3F7) [H00003654-M01] - Analysis of IRAK1 transfected lysate using anti-IRAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRAK1 MaxPab rabbit polyclonal antibody.

Sandwich ELISA: IRAK1 Antibody (3F7) [H00003654-M01] - Detection limit for recombinant GST tagged IRAK1 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
• Clone: 3F7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IRAK1 Antibody (3F7) Summary

• Immunogen: IRAK1 (AAH54000, 530 a.a. ~ 693 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SSTGRAHSGAAPWQPLAAPSGASAQAAEQLQRGPNQPVESDESLGGLSAALRSWHLTPSCPLDPAPLREAGCPQGDTAGESSWGSGPGSRPTAVEGLALGSSASSSSEPPQIIINPARQKMVQKLALYEDGALDSLQLLSSSSLPGLGLEQDRQGPEESDEFQS
• Localization: interleukin-1 receptor complex
• Specificity: IRAK1 - interleukin-1 receptor-associated kinase 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IRAK1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IRAK1 Antibody (3F7)

• EC 2.7.11
• EC 2.7.11.1
• Il1rak
• interleukin-1 receptor-associated kinase 1
• IRAK1
• IRAK-1
• IRAKPelle homolog
• Pelle
• Plpk

Background

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRAK1
• Entrez:
  • 3654(Human)
• OMIM:
  • 300283(Human)
• Uniprot:
  • AAH54000()