IQGAP2 Antibody - Azide and BSA Free

Western Blot: IQGAP2 Antibody [NBP3-04682] - Analysis of extracts of various cell lines, using IQGAP2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit

Immunohistochemistry-Paraffin: IQGAP2 Antibody [NBP3-04682] - Immunohistochemistry of paraffin-embedded Rat kidney using IQGAP2 Rabbit pAb (NBP3-04682) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: IQGAP2 Antibody [NBP3-04682] - Immunohistochemistry of paraffin-embedded Human breast cancer using IQGAP2 Rabbit pAb (NBP3-04682) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IQGAP2 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit IQGAP2 Antibody - Azide and BSA Free (NBP3-04682) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1330-1440 of human IQGAP2 (NP_006624.2). QQEVDHATDMVSRAMIDSRTPEEMKHSQSMIEDAQLPLEQKKRKIQRNLRTLEQTGHVSSENKYQDILNEIAKDIRNQRIYRKLRKAELAKLQQTLNALNKKAAFYEEQIN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IQGAP2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for IQGAP2 Antibody - Azide and BSA Free

• IQ motif containing GTPase activating protein 2
• IQGAP2
• ras GTPase-activating-like protein IQGAP2

Background

The IQGAP2 gene encodes a member of the IQGAP family. The protein contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several sig

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: IQGAP2