Intelectin-2 Antibody (874029)

Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Intelectin-2 Monoclonal Antibody (Catalog # MAB8004) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Intelectin-2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intelectin-2 was detected in formalin fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human Intelectin-2 Monoclonal Antibody (Catalog # MAB8004) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Paneth cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 874029
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Intelectin-2 Antibody (874029) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Intelectin-2
  Met1-Tyr324
  Accession # Q8WWU7
• Specificity: Detects human Intelectin-2 in ELISA. In Direct ELISA, no cross-reactivity with recombinant human (rh) Intelectin-1, recombinant mouse (rm) Intelectin-1, or rmIntelectin-2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITLN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Intelectin-2 Antibody (874029)

• HL-2
• intelectin 2
• Intelectin2
• Intelectin-2
• Intelectin-b
• Itln1b
• ITLN2
• Itlnb
• UNQ2789/PRO7179

Background

Intelectin-2 (ITLN-2; also endothelial lectin 2 and HL-2) is a 37-41 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules.  It is secreted by intestinal Paneth cells, and appears to participate in fungal microbe recognition.  The human ITLN-2 precursor is 325 amino acids (aa) in length (SwissProt #:Q8WWU7).  It contains a 26 aa signal sequence and a 299 aa mature region.  Although mouse ITLN-2 is reportedly GPI-linked, and mouse and human ITLN-2 show considerable C-terminal aa identity, it is not known if human ITNL-2 is also GPI-linked.  There is one fibrinogen-related domain in the mature molecule (aa 44-267).  One potential splice variant is reported that shows a premature truncation after Gly264.  Over aa 29-324, human ITLN-2 shares 79% aa sequence identity with mouse ITLN-2/ITLN-1b, and 85% aa sequence identity with human ITLN-1.      

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Intelectin-2 Antibody (MAB8004) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 04/09/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Intestine
• Species: Human
• Lot: 874029

Comments

• Comments: Intestine tissue was stained at 5 ug/mL overnight at 4C

Bioinformatics

• Gene Symbol: ITLN2
• Uniprot:
  • Q8WWU7(Human)